CytoFlex Flow Cytometer Application Notes

Nanoparticle Detection

The advancement of flow cytometry into nanoparticle scale resolution, makes it possible to ask questions previously left to speculation. Several fundamental capabilities of flow cytometry make it an attractive platform for studying nanoparticles such as extracellular vesicles. That is the ability to detect large numbers of events, and discrimination of rare events, while simultaneously collecting information on phenotypic expression.

The CytoFLEX Platform of flow cytometers features the capability to measure side scatter off of the violet as well as the blue laser. This increases the range of particles that can be detected and analyzed within the sample. The smaller violet (405 nm) wavelength will result in more orthogonal light scattering at any given particle size than the blue (488 nm) wavelength. The use of violet light will help to amplify the differences in the refractive indices between the particles and their surrounding media, and in turn increases the ability to detect particles with a lower refractive index, such as exosomes, microvesicles and silica nanoparticles.

EVERY Event Matters

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