CytoFlex Flow Cytometer Application Notes

CytoFlex Flow Cytometer Brochure5
CytoFLEX Platform 96-well Plate Sample Loaders25
Technical Note26
Successfully importing CytoFLEX CytExpert software data into third party software programs27
CytoFLEX Research Cytometer Event Rate Settings31
CytoFLEX Flow Cytometers with CytExpert 2.0 Acquisition and Analysis Software - Support for Compliance with 21 CFR Part 1137
Expanding the Useful Spectrum for Flow Cytometry: Increasing the Nimber of Parameters Available Without Adding Compensation Complexity41
Cell Viability and Vitality Application Notes57
Viability Assessment of Cell Cultures using the CytoFlex58
Comparing Viability Measurements on Cell Lines Utilizing the CytoFLEX Flow Cytometer61
Measuring Proliferation in Leukemia Cell Lines via Carboxyfluorescein Succinimidyl Ester (CFSE) upon Treatment with CytostaticConcentrations of Cytosine Arabinoside (Ara-C).63
Fully-Automated Cellular Analysis by Flow Cytometry67
Immunophenotyping Application Notes69
6 Color Panel for Staining Human Peripheral Blood Lymphocytes70
T Cell Receptor Characterization of PBMCs Using the CytoFlex72
Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice74
Phenotypic characterizing of human Natural Killer (NK) cell populations in peripheral blood76
Identification of NK subsets in mice79
Identification of Natural Killer Cells in Rat Placenta Model of Preeclampsia82
Competitive Bone Marrow Transplantation in C57/BL6 Mice84
Surface staining of mouse splenocytes and peripheral blood cells87
Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DuraClone dry reagent technology91
Characterizing Human CD4+ T cell Populations by Intracellular Cytokine Profile Using Flow Cytometry94
Beadless Absolute Counting Application of the unique properties of the peristaltic pump fluidic based system for volumetric cell counting99
18-Color Human Blood Phenotyping Made Easy with Flow Cytometry106
Functional Assays Application Notes98
Flow Cytometric Quantification to Assess Dorsal Endocytosis115
Phagocytosis of Bacteria in a Whole Blood Assay: The Influence of Opsonization on Monocyte and Granulocyte Phagocytosis118
Single Cell Analysis of Autophagy124
Neutrophil Oxidative Burst Assay: A Dihydrorhodamine (DHR) based testing of Chronic Granulomatous Disease (CGD)with CytoFlex Flow Cytometer128
The unique peristaltic sample delivery system of the CytoFLEX analyzer enables optimized measurements of transient changes inintracellular calcium in cells following agonist activation.132
Flow Cytometric Measurement of CD62P Platelet Activation136
Analysis of plant genome sizes using flow cytometry: a case study demonstrating dynamic range and measurement linearity140
Microbiology Application Notes123
Detecting and counting bacteria with the CytoFLEX research flow cytometer: I-Characterization of a variety of gram-negative bacteria153
Detecting and counting bacteria with the CytoFLEX research flow cytometer: II-Characterization of a variety of gram-positive bacteria157
Counting Eschericia coli Using the CytoFLEX Research Flow Cytometer162
Flow Cytometric Analysis of Bacterial Protein Aggregation During Expression of Foreign Proteins164
Flow Cytometric Analysis of auto-fluorescent cells found in the marine demosponge Clathria prolifera168
Flow Cytometric Approach to Probiotic Cell Counting and Analysis172
Nanoparticles Application Notes150
Beckman Coulter CytoFLEX Violet SSC: an Alternative to FSC PMT or Fluorescence in the Detection of Extracellular Vesicles179
How to Use Violet Side Scatter to Detect Nanoparticles on the CytoFLEX Flow Cytometer187
Set-Up of the CytoFLEX* for Extracellular Vesicle Measurement193
Setting up the Beckman Coulter CytoFLEX for Detection of Extracellular Vesicles200
Red Blood Cell (RBC) Microparticle Analysis by Flow Cytometry205
CytoFlex Technical Specifications180
CytoFLEX Violet-Blue-Red (V-B-R) Series209
CytoFLEX S Violet-Blue-Yellow Green-Red (V-B-Y-R) Series.211
CytoFLEX S Near UV-Violet-Blue-Red (N-V-B-R) Series213
CytoFLEX S Violet-Blue-Red-Infrared (V-B-R-I) Series215
CytoFLEX S Near UV-Violet-Blue-Yellow Green (N-V-B-Y) Series217
CytoFLEX LX Near UV-Violet-Blue-Yellow Green-Red-Infrared (N-V-B-Y-R-I) Series219
CytoFLEX LX UV-Violet-Blue-Yellow Green-Red-Infrared (U-V-B-Y-R-I) Series221

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