CytoFlex Flow Cytometer Application Notes

Figure 6. Compensation evaluation of fluorescent dye spillover into the IR channels. AF790-stained VersaComp beads were run on the CytoFLEX S Blue-Red-Violet-Infrared Flow Cytometer (left column). The fluorescence measurement of AF790-stained sample in the AF790 IR channel is compared to all other channels. Very little spillover of AF790 dye is observed in other channels except for the PF840 IR channel. PF840-stained cells was run on the CytoFLEX S Blue-Red-Violet-Infrared Flow Cytometer and the fluorescence measurement in the PF840 channel is compared to all other channels (right column). The PF840 dye does not produce significant spill over into other channels except for the AF790 IR channel.

Fluorochrome

AF790 Channel

PF840 Channel

APC

APC-A700

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