CytoFlex Flow Cytometer Application Notes

250

850

Indo-1 (calcium) Indo-1 (no calcium) DAPI Alexa 350 Hoechst 33342 Propidium Iodide Cascade Blue Pacific Blue Alexa 430 Cascade Yellow Krome Orange Pacific Orange AmCyan FITC eGFP Alexa 488 PE ECD (PE-Texas Red) PerCP

640nm, 645nm laser 592nm laser 560nm laser 532nm laser 488nm laser 405nm laser 355nm laser

PC5 (PE-Cy5)

PC5.5 (PE-Cy5.5)

PC7 (PE-Cy7) PE

PE-Alexa 610 PE-Alexa 647 7-AAD ECD (PE-Texas Red) PC5 (PE-Cy5)

PC5.5 (PE-Cy5.5)

PC7 (PE-Cy7) mCherry

Ds-Red mPlum PE ECD (PE-Texas Red) PC5 (PE-Cy5)

PC5.5 (PE-Cy5.5)

PC7 (PE-Cy7) Texas Red APC APC-Cy7 APC-Alexa700 APC-Alexa750

Alexa 647 Alexa 700 Alexa 750 Alexa 633 APC Alexa 647 Alexa 660 Alexa 680 Alexa 700 Alexa 750 APC-Cy7

APC-Alexa700 APC-Alexa750

250

850

Figure 2. Emission Spectra for Common Fluorochromes. The visible spectrum is 390 to 700 nm and a variety of fluorochromes are available across these wavelengths. Expanding the number of markers that can be interrogated simultaneously requires expanding the useful spectrum at the edges.

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