Genomics Application Notes

PCR Reaction Setup Application

3. Supports both prepared PCR master mix and master mix made from individual PCR components (Figure 3) .

4. Keeps track of tip usage and alerts the user if more tip boxes are needed (Figure 4) .

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Figure 3 . Master Mix can be made from individual PCR components, i.e., AmpliTaq DNA polymerase, dNTP, MgCl2,Triton X- 1 00,Waters and Primers. All PCR components can be transferred from tubes of source labware to the tube of destination labware.

5. Allows saving and importing of setup conditions to repeat prior setup processes (Figure 5) .

6. Provides standard pipetting templates and techniques which can also be customized by users. 7. Provides sample tracking report upon run completion.

Figure 4. Tip usage is automatically tracked and calculated per number of samples processed.

Figure 5. Click the “Save” button to save the setup condition. Click the “Load” button to import a saved file for repeat PCR reaction setup conditions.

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B2012-13913-DG-1K