Genomics Application Notes

Figure 5. High quality reads for Read 1 and 2 of the Ilumina HiSeq 2500 Run.

Figure 6. Total base counts for each library, combining Read 1 and Read 2 results

Highly Efficient Interrogation of the Transcriptome The goal of every transcriptome sequencing project is to obtain results that provide the most information on all RNA transcripts, with a minimum of extraneous sequence information. The results from the human libraries constructed using the Biomek FX P and the TruSeq Stranded mRNA Kit are highly targeted to the polyA transcriptome, with very low percentages of adapter dimers and sequence that do not align to the polyA transcriptome, or that align with ribosomal, 5s RNA, or mitochondrial sequences (Table 3).

Table 3. Key Sequencing Results Percentage (%) of PF Reads*

UHR LIBRARY

READ % ALIGNED

% UNALIGNED % HUMRIBOSOMAL % ADAPTER DIMER

UHR2-1 ssRNA TruSeq Library

1

95.6%

4.4%

0.9%

0.2%

UHR2-2 ssRNA TruSeq Library

1

95.5%

4.5%

1.2%

0.3%

UHR2-3 ssRNA TruSeq Library

1

95.9%

4.1%

0.9%

0.1%

UHR2-4 ssRNA TruSeq Library

1

95.1%

4.9%

0.9%

0.3%

UHR2-5 ssRNA TruSeq Library

1

95.2%

4.8%

1.1%

0.2%

UHR2-7 ssRNA TruSeq Library

1

95.5%

4.5%

13.0%

0.2%

UHR2-8 ssRNA TruSeq Library

1

95.5%

4.5%

2.4%

0.2%

UHR2-9 ssRNA TruSeq Library

1

94.4%

5.6%

1.1%

0.5%

UHR2-1 ssRNA TruSeq Library

2

93.3%

6.7%

0.9%

0.2%

UHR2-2 ssRNA TruSeq Library

2

93.4%

6.6%

1.2%

0.3%

UHR2-3 ssRNA TruSeq Library

2

94.0%

6.0%

0.9%

0.1%

UHR2-4 ssRNA TruSeq Library

2

92.8%

7.2%

0.9%

0.3%

UHR2-5 ssRNA TruSeq Library

2

92.7%

7.3%

1.1%

0.2%

UHR2-7 ssRNA TruSeq Library

2

93.5%

6.6%

13.0%

0.2%

UHR2-8 ssRNA TruSeq Library

2

93.2%

6.9%

2.4%

0.2%

UHR2-9 ssRNA TruSeq Library

2

92.1%

7.9%

1.1%

0.6%

B2013-14332 IB-18319A