Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

CD207 ( Langerin ) CD207 (Langerin) is an antigen described as selectively expressed by Langerhans cells (LC), a subset of immature dendritic cells (DC) formed in epidermis and mucosa. Langerin is a 40 kDa glycoprotein predominantly N-glycosylated which contains no disulfide bond. Its expression, restricted to the Langerhans cell lineage, is demonstrated at both the surface membrane and in the cytoplasm. Several lines of evidence indicate that Langerin is a marker specific for the acquisition of the Langerhans cell phenotype. It is down-regulated beyond the LC maturation stage. Langerin is not involved in antigen delivery into the MHC class II pathway but may play a role in antigen capture. Langerin is present only in epidermis and airway epithelia. Langerin is not detected in purified DCs isolated from peripheral blood, lymph nodes or thymus. CD1a+ DCs derived from CD34+ cord blood cell cultures in the presence of GM-CSF and TNF- α express Langerin between day 8 and 12. DCs derived from peripheral blood monocytes cultured with GM-CSF and IL-4 do not express Langerin. Isotype: IgG1 Mouse The DCGM4 antibody stains exclusively immature DCs of the LC lineage. Both intracytoplasmic and surface membrane staining is observed. DCGM4 acts as a ligand for Langerin, triggering rapid endocytosis within 20 minutes at 37 o C. The antibody has been used in intracellular immunofluorescence analyzed in confocal microscopy. The DCGM4 antibody has been used to determine the differentiation of the CD1a + /CD11c + subset of blood DCs into LCs, and in the characterization of DCs infiltrating breast carcinoma tissue. Clone: DCGM4

NIH-LG.13 Cell line.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM3577

2 mL

Liquid

ASR

IOTest

GMP

CD235a ( Glycophorin A ) The CD235a antigen, also called Glycophorin A, is a transmembrane, single-pass glycoprotein that contains carbohydrate, mostly in the form of sialic acid. CD235a has a molecular weight of 10 kDa. It is expressed by erythroid precursors and erythrocytes. Glycophorins A carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, and may also serves as receptor for cytokines and pathogens, including the malaria parasite, Plasmodium falciparum and some viruses, including influenza virus and hepatitis A virus.

Lysed normal whole blood sample

Clone: 11E4B-7-6 (KC16) Isotype: IgG1 Mouse The 11E4B-7-6 (KC16) antibody binds to the amino acid sequence 27-39 of glycophorin A and has no reactivity to glycophorin B. It reacts with immature erythrocytes including erythroblasts, proerythroblasts and reticulocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM2212U IM2211U A71564 A89314

2 mL 2 mL 1 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

PE

PC7

APC-A750

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 98 US Clinical Diagnostics Catalog