Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

CD134 ( OX 40 ) The CD134 antigen is a 35 kDa, type-1 transmembrane glycoprotein member of the tumor necrosis factor superfamily (TNFRSF) and is allocated number 4. This antigen was originally named OX40 from the first antibody that led to the discovery of this antigen on activated rat CD4+ T cells. In humans, CD134 is expressed at high levels on activated CD4+ T cells and virally stimulated CD4+ T cells. Its ligand, known as gp34, is a type-2 transmembrane glycoprotein member of the tumor necrosis factor (TNF) superfamily, expressed on activated T and B cells, dendritic cells and macrophages. The CD134 antigen, through its interaction with adaptor proteins TRAF2, TRAF3 and TRAF5, is a costimulatory signal transducer of T-cell receptor- mediated activation that has been shown to activate NF- κ B, a signaling factor involved in transcription of survival genes. Thus, CD134 engagement with its ligand induces clonal expansion and survival of CD4+ cells during primary responses, and results in the accumulation of increasing numbers of memory cells with time. Finally, CD134 has been shown to be involved in the T cell adhesion to endothelium.

Lysed normal whole blood sample.

Form Clone: Ber-ACT35

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

APC

B30629

0.5 mL

Liquid

ASR

IOTest

GMP

CD135 ( flt3/flk2 ) The CD135 antigen, also called FLT3 or FLK2 or STK1, is an integral transmembrane glycoprotein of 130 kDa to 150 kDa, which belongs to the class III receptor tyrosine kinase (RTK) family. These receptors directly bind extracellular ligands and transduce regulatory signals through intracytoplasmic tyrosine kinase activity. The CD135 antigen is expressed on myelomonocytic and primitive B cell progenitors. On normal bone marrow cells, expression of CD135 can be found on CD34-positive as well as CD34-negative cells. Most of the CD34bright, CD135-positive cells co-express CD117 at high levels. They may represent early cycling, not quiescent stem cells. CD135-positive cells in the CD34 low and CD34-negative populations do not co-express CD117 molecule and may represent B-lymphoid precursors.

JEA2 Cell line.

Clone: SF1.340 Isotype: IgG1 Mouse The SF1.340 antibody is specific for an extracellular domain of CD135. This antibody does not compete with the FLT3 ligand for binding to FLT3.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2234U

2 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 85 US Clinical Diagnostics Catalog