Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

CD85k ( ILT3 ) The CD85k antigen (ILT3) is a member of Immunoglobulin (Ig)-Like Transcripts (ILT), also known as Leucocyte Ig-like Receptors (LIR) and Monocyte / Macrophage Ig-like Receptors (MIR). ILT are expressed on monocytes, macrophages, dendritic cells (DCs) and granulocytes, except ILT2, which is expressed by both myeloid and lymphoid cells: NK, T cell subsets and all peripheral B cells. All ILT receptors except (ILT6) are transmembrane proteins and have been classified in 3 types: Inhibitory: ILT2, 3, 4, 5, LIR8; Activating: ILT1, LIR6a, 7, LIR8, with a cytoplasmic tail associated to FcR γ ; Soluble: ILT6. The CD85k antigen (otherwise known as LIR-5, HM18) is a transmembrane protein of 60 kDa, constitutively phosphorylated. Unlike ILT2 and ILT4, ILT3 does not bind to HLA-A, -B and -G1 molecules. The ligand of ILT3 is unknown, however research studies point out the following functional properties: ILT3 is involved in antigen uptake and presentation; ILT3 negatively regulates Antigen Presenting Cell (APC) functional responses triggered via stimulatory receptors such as CD11b, CD16, MHC-class II. The transmission of a negative signal via ITIMs may be one of the inhibitory mechanisms, ranging from temporary to permanent inactivation, that control homeostasis, and immunological tolerance. It has been shown that ILT3, unregulated on monocytes and DCs by CD8+CD28- T suppressor cells, renders these APC tolerogenic. Isotype: IgG1 Mouse The ZM3.8 antibody immunoprecipitates a protein of 55 kDa from ILT3-transfected T cells and a protein of 60 kDa from monocytes. CD86 The CD86 antigen (B7-2, B70) is a single-chain trans-membrane glycoprotein, structurally similar to CD80. Its molecular weight is 80 kDa, under reducing conditions. The extracellular region is composed of one V-type and one C-type Ig-like domains. There are 8 potential sites for N-glycosylation. The cytoplasmic tail has 3 potential sites for protein kinase C phosphorylation. CD86 shares with the CD80 molecule the same co-receptors on T cells, CD28 and CD152 (CTLA-4). CD86 binds to CD152 with a 20-100-fold higher affinity than to CD28. CD86 is expressed constitutively by interdigitating dendritic cells, and at lower level by peripheral blood dendritic cells. It is strongly expressed by monocytes. On lymphocytes, CD86 appears as a B-cell activation antigen. CD86 is preferentially expressed by memory B cells and germinal center B cells, but not on plasma cells. Its expression can be up-regulated by activation through surface immunoglobulin, CD40, MHC class II molecules or by PMA with ionomycin. T cells activated by CD3 ligation have also been reported to express CD86. Clone: ZM3.8 Part# Format Size Status Line Quality Standard PC7 IOTest ASR A46529 Liquid 1 mL GMP

Lysed normal whole blood sample.

Form

Lysed normal whole blood sample.

Form Clone: HA5.2B7

Isotype: IgG2b Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

IM2729U B30647 B30648 B30646

2 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

PC5.5

0.5 mL 0.5 mL 0.5 mL

PC7

APC-A750

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 78 US Clinical Diagnostics Catalog