Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

CD63 The CD63 antigen, also known as lysosomal membrane associated glycoprotein 3 (LAMP3), is a member of the tetraspanin (TM4SF) family. Nearly 20 genes encode tetraspanins whose main structural characteristics is their four transmembrane domains. CD63, as other tetraspanins (CD9, CD81, CD82), has recently been reported as forming complexes with VLA-3 and phosphatidylinositol 4-kinase, with VLA-6, CD11/CD18 and tyrosine kinase. CD63 was first described in granules of resting platelets and on the surface membrane on activated platelets. CD63 was later identified as granulophysin, a platelet dense granule glycoprotein. The molecular weight of the platelet form of CD63 ranges from 40 to 55 kDa. CD63 has been found to be identical to the ME491 antigen expressed by melanoma cells. It is also found on monocytes / macrophages and is weakly expressed by resting granulocytes, T lymphocytes and B lymphocytes. CD63 antigen, present in azurophilic granules of non-stimulated neutrophils, is strongly expressed on the surface of neutrophils after activation. Studies have shown that CD63 may be a marker for granule release. This phenomenom is also reported for basophils. Isotype: IgG1 Mouse The CD63 antibodies inhibit adhesion of neutrophils to activated endothelium. CLBGran/12 was used as a CD63 reference monoclonal antibody during HLDA 6. Clone: CLB-Gran/12

Lysed normal whole blood sample.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1165U IM1914U

2 mL 2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

PE

CD64 CD64 is an integral membrane glycoprotein known as an Fc receptor that binds monomeric IgG-type antibodies with high affinity. It is also known as Fc-gamma receptor 1 (Fc γ RI) or FCRI. After binding IgG, CD64 interacts with an accessory chain known as the common γ chain, triggering cellular activation. Structurally, CD64 is composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type used to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on macrophages and monocytes. CD64 is expressed on early myeloid cells and in IFN γ and G-CSF activated polymorphonuclear neutrophils (PMNs).

Lysed normal whole blood sample.

Clone: 22 Isotype: IgG1 Mouse The 22 antibody shows especially high affinity binding to human mononuclear phagocytes.The epitope recognized by this antibody is distinct from the IgG binding site.

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B19718

0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR ASR ASR ASR ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest

GMP GMP GMP GMP GMP GMP GMP

FITC

IM1604U IM3601U A98434 IM3606U B06025 A89308

2 mL 2 mL

PE

ECD PC5 PC7

0.5 mL

1 mL

0.5 mL 0.5 mL

APC-A750

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 71 US Clinical Diagnostics Catalog