Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

CD40 The CD40 antigen is a 44-48 kDa type I integral membrane protein of the tumor necrosis factor receptor (TNFR) superfamily. This antigen is found on B cell lines, is strongly expressed by interdigitating cells (IDC), basal epithelial cells, and is also present on macrophages, some endothelial cells, and follicular dendritic cells. It is a pan-B marker, absent only from terminally differentiated plasma cells. CD40 is implicated in the process of B cell selection in the germinal centre. Studies demonstrated that CD40 monoclonal antibodies (mAbs) induce strong homotypic adhesions in resting B cells and, together with interleukin-4 (IL-4) maintain the cell cycle of blasts of the B lineage. They also can promote the switch to IgE secretion. Activated B cells via CD40 antigen in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. The CD154, (CD40 ligand) is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion. CD154 is also expressed on activated platelets and triggers an inflammatory reaction of endothelial cells.

Lysed normal whole blood sample.

Clone: MAB89

Isotype: IgG1 Mouse

Co-culture with MAB89 and IL-4 leads to long term B cell proliferation.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM1936U B20025 B30636

2 mL

Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest

GMP GMP GMP GMP

PC5.5

0.5 mL 0.5 mL 0.2 mg

APC

UNLB

IM1374

Freeze-dried

-

CD41 The CD41 antigen (platelet GPIIb; IIb integrin) is a transmembrane glycoprotein composed of two chains GPIIb α (120 kDa) and GPIIb β (23 kDa) linked by one disulfide bond. CD41 is always non-covalently associated with CD61 (platelet GPIIIa, β 3 integrin), to form the GPIIb-IIIa (CD41/CD61) complex. CD41 is expressed by platelets, megakaryocytes and a small subset of CD34+ cells, suggesting that CD41/CD61 is one of the earliest markers of the megakaryocytic lineage. The resting form of the CD41/CD61 complex binds to immobilized fibrinogen and upon platelet activation, the complex becomes a receptor for soluble fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. It is involved in platelet aggregation.

HEL cell line

Clone: P2 Isotype: IgG1 Mouse The P2 antibody reacts with the α chain of CD41a (GPIIb α ) in the intact complex with GPIIIa but not with the GPIIb or GPIIIa separately. This antibody blocks binding to fibrinogen and inhibits platelet aggregation induced by thrombin, collagen and ADP. The P2 antibody was used as a CD41 reference mAb during HLDA 6.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM0649U IM1416U

2 mL 2 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

GMP GMP GMP

PE

APC

B16894

0.5 mL

Clone: SZ22 Isotype: IgG1 Mouse The SZ22 monoclonal antibody reacts with the α chain of CD41 (GPIIb α ) on platelets and megakaryocytes. This monoclonal does not inhibit platelet aggregation and secretion induced by collagen, arachidonic acid, and thrombin. It does not inhibit fibrinogen binding to human platelets induced by ADP, arachidonic acid and PAF.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1756U

2 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 60 US Clinical Diagnostics Catalog