Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

APO2.7 ( 7A6 ) The APO2.7 antigen (also named 7A6 antigen) is a 38 kDa protein localized on the membrane of mitochondria whose expression appears to be restricted to cells undergoing apoptosis. The APO2.7 antigen can be detected after apoptosis induction via CD95/Fas ligation, irradiation or drug treatment. Expression of APO2.7 appears as an early event in the apoptosis process. Normal viable cells are negative or weakly positive for APO2.7. Research studies have shown that less than 2% of peripheral T cells from normal donors expressed the APO2.7 antigen. Some level of APO2.7 expression associated with an on-going apoptotic process has been demonstrated in activated T cells.

Apoptotic Jurkat cell line

Clone: 2.7A6A3 (APO2.7) Isotype: IgG1 Mouse The APO2.7 antibody can be used for the direct quantitation of apoptotic cells by flow cytometry, after permeabilization with digitonin. Use of APO2.7 allows for precise monitoring of the early and late cellular responses during apoptosis.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2088U

2 mL

Liquid

ASR

IOTest

GMP

CD1a CD1a is a member of the lipid antigen presenting molecules family Like the other CD1 proteins (group 1: CD1a, CD1b, CD1c and group 2: CD1d, CD1e), CD1a is a transmembrane protein of 49 kDa, noncovalently associated with β 2-microglobulin. As other group 1 CD1 antigens, CD1a is found on professional antigen-presenting cells (APC) such as dendritic cells, particularly on Langerhans cells and their direct circulating precursors. It is expressed by those cells related to dermal dendritic cells, derived from monocytes cultured for 6 days in the presence of GMCSF and IL-4. The CD1a antigen is also present on a B cell subset. It is also strongly expressed by cortical thymocytes. CD1a antibodies are useful in the definition of the thymic stage of T cells.

MOLT4 cell line

Clone: BL6

Isotype: IgG1 Mouse

The BL6 antibody recognizes the C epitope of the CD1a molecule.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

B25330 IM1942U IM3610 IM3645

1 mL 2 mL 1 mL 1 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

PE

PC5 APC

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 33 US Clinical Diagnostics Catalog