Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

IgD IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It exists in a transmembrane and a soluble form. IgD is, together with IgM, the first antibody isotypes expressed during B cell ontogeny. Bone marrow B cell precursors acquire surface IgM after assembling heavy (H) and light (L) chain variable region exons from prototypic variable (V), diversity (D) and joining (J) gene segments through an antigen-independent process. After leaving the bone marrow to colonize secondary lymphoid organs, B cells acquire surface IgD of the same specificity as surface IgM through alternative splicing of a pre-messenger RNA comprising V(D)J and both heavy chain constant μ (C μ ) and C δ exons.

Pre-washed lysed normal whole blood sample.

Clone: IA6-2

Isotype: IgG2a Mouse

The IA6-2 monoclonal antibody binds specifically to the heavy chain of human IgD.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

B30652 B30653 B30651

50 tests 50 tests 50 tests

Liquid Liquid Liquid

RUO RUO RUO

IOTest IOTest IOTest

GMP GMP GMP

PE

APC

IgM The anti-IgM antibody binds to circulating IgM as well as to IgM antibodies bound to the Fc μ R. The Fc μ R is a transmembrane single sialoglycoprotein of 60 kD with O-linked oligosaccharides. It contains an extracellular Ig-like domain homologous to two other IgM-binding receptors (polymeric Ig receptor and Fc α / μ R) but exhibits an exclusive Fc μ -binding specificity. Unlike other FcRs, the major cell types expressing Fc μ R are adaptive immune cells, including B and T lymphocytes. The Fc μ R can be expressed as a cell surface activation antigen throughout the pre-B and B cell stages in differentiation. Receptor expression is not directly linked with IgM production, as both μ - pre-B cells and isotype-switched B cells may express the Fc μ R. The Fc μ R is thus the third member of a family of Fc receptors expressed on B-lineage cells, and its preferential expression on activated B cells suggests a potential role in the response to antigens.

Pre-washed lysed normal whole blood sample.

Clone: SA-DA4

Isotype: IgG1 Mouse

The SA-DA4 monoclonal antibody binds specifically to mu heavy chain of Human Immunoglobulin (IgM).

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B30656 B30655 B30657 B30654

50 tests 50 tests 50 tests 50 tests

Liquid Liquid Liquid Liquid

RUO RUO RUO RUO

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

FITC

PE

APC

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 194 US Research Flow Cytometry Catalog