Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

DAP12 ( DNAX / KARAP / TYROBP ) The DAP12 antigen (DNAX activating protein of 12 kDa) is a type I transmembrane glycoprotein which is also known as KARAP (killer cell activating receptor-associated protein) or TYROBP (tyrosine kinase binding protein). It is encoded by a gene located on the chromosome 19q13 and contains an intracytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). The DAP12 antigen associates with a large family of receptors in hematopoietic cells conferring to this antigen a broad range of intracellular signaling functions Interestingly, it has been shown to both potentiate or inhibit leukocytes activation. When associated with KIRs (stimulatory killer Ig-like receptors), the adaptor molecule DAP12 functions as an activation unit leading to the T cell activation. It has also been shown that the activation of the syk/ZAP70 tyrosine kinases by DAP12 plays a role in the stimulation of NK cells and T cells. On the contrary, the DAP12 association with other receptors such as Fc receptor γ -chain and CD3 ζ may lead to the regulation of T cell immune response. Isotype: IgG1 Rat The H10E12F4 monoclonal antibody recognizes monocytes, neutrophils, dendritic cells, NK cells and to a lesser extend basophils, and B lymphocytes. It does not recognize CD4 and CD8 T cells or B cells. Clone: H10E12F4

RBL-KAR-KARAP Cell line.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

A78886

100 tests

Liquid

RUO

IOTest

GMP

Helios Helios belongs to the Ikaros transcription factor family. Members of this family are DNA binding proteins containing two-zinc finger N terminal domains and protein binding domain. Helios expression is restricted to the T lymphocytes lineage. Helios is suggested to play an important role in CD4+CD25+FoxP3+ regulatory cells. It has been shown that Helios up-regulates the expression of FoxP3 expression by binding to the FoxP3 promoter. Helios is a highly conserved transcription factor that has 97% sequence homology between human and mice.

Lysed normal whole blood sample.

Clone: 22F6

Isotype: IgG Hamster

The clone 22F6 reacts with human and mouse Helios.

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B49311

50 tests

Liquid

RUO

IOTest

GMP

HIV-1 core antigen

Clone: KC57 Isotype: IgG1 Mouse The KC57 antibody identifies the 55, 39, 33 and 24 kDa proteins of the core antigens of the human immunodeficiency virus type 1 (HIV-1). The 55 kDa protein is the precursor protein for the core antigen. The 39 and 33 kDa proteins are intermediate products and the 24 kDa protein is the core protein.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

6604665 6604667

100 tests 100 tests

Liquid Liquid

RUO RUO

GMP GMP

COULTER CLONE

RD1

COULTER CLONE

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 192 US Research Flow Cytometry Catalog