Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD209 ( DC-SIGN ) The CD209 antigen, also named dendritic cell (DC)-specific-intracellular adhesion molecule 3 (ICAM-3)-grabbing non-integrin (DC-SIGN), is a 44 kDa type II transmembrane C-type lectin with a single carbohydrate recognition domain (CRD). Like other C-type lectin members, CD209's extracellular structure is able to bind mannose residues in a Ca++ dependent manner. Highly specific to DCs, CD209 plays a key role in the primary immune response as receptor / processor for a variety of ligands, including: ICAM-3: initiation of DC-T cell immunological synapse; ICAM-2: transendothelial DC migration; HIV-1 gp120: capture of HIV-1 and transpresentation to CD4+ T cells; CMV envelope glycoprotein B: capture of CMV; Unknown ligands: capture of Ebola virus, and of Leishmania amastigotes. CD209 is expressed by immature DCs in peripheral tissues, with the noticable exception of Langerhans cells. It is also expressed by DCs in lymphoid tissues, and by certain macrophages. In peripheral blood, a percentage of plasmacytoid DCs (or DC2) may also express CD209. It is totally absent from monocytes, and an observed up-regulation of CD209 expression on monocyte-derived DCs correlates with the down-regulation of CD14. Monocyte-derived macrophages may express CD209, only when cultured in the presence of IL-13. Isotype: IgG1 Mouse The AZND1 monoclonal antibody (mAb) is useful for many purposes, including flow cytometric analysis of CD209- expressing cells, immunohistochemical analysis of CD209 + cells on frozen tissue sections, immunoprecipation of CD209 antigen, and investigation of its functions, due to the blocking or inhibiting properties of AZND1 mAb. The AZND1 mAb to DC-SIGN has been assigned to the CD209 cluster of differentiation during HLDA 7. Clone: AZND1

K562-SIGN Cell line.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

A07407 A07406

100 tests

Liquid Liquid

RUO RUO

IOTest

GMP GMP

UNLB

0.2 mg

-

CD274 ( PD-L1 ) The CD274 antigen is known as Programmed Cell Death 1 ligand 1 (PDCD1LG1 or PD-L1) or B7-H1. It is encoded by a gene located on the human chromosome 9p24. Immune cell function implicates important modulators known as cosignaling receptors. On T cells, the cosignaling receptors belong to either the immunoglobulin (CD28-like) or TNF receptor (TNFR) superfamilies. The CD28 immunoglobulin-superfamily is composed of coinhibitory (CD28, ICOS) and costimulatory receptors. Programmed Death- 1 (PD-1) is one of the three coinhibitory receptors with Cytotoxic T Lymphocyte Antigen 4 (CTLA4) and CD272. CD274 antigen is one of the two ligands, with PD-1 ligand 2 (PD-L2), for PD-1 and belongs to the B7 family. The PD-1 - PD-L1 interaction plays a critical role in regulating T cell activation and tolerance. In particular, it plays a role during development by inhibiting the TCR-mediated positive selection of thymocytes. It has also been shown to be implicated in the regulation of CD4 positive T cells proliferation. CD274 antigen is expressed on immature dendritic cells (iDC), mature dendritic cells (mDC), IFN- γ -treated monocytes, and follicular dendritic cells.

Clone: PD-L1 Isotype: IgG1 Mouse The PD-L1 monoclonal antibody recognizes dendritic cells, T cells and follicular dendritic cells. It has been assigned to the CD274 cluster of differentiation during the 8th HLDA Workshop on Human Leukocyte Differentiation Antigens, held in Adelaide, Australia, in 2004.

Form

Size

Format

Part#

Status

Line Quality Standard

PC7

A78884

50 tests

Liquid

RUO

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 189 US Research Flow Cytometry Catalog