Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD158b1/b2,j The CD158b1, CD158b2 and CD158 j molecules are monomeric integral membrane glycoproteins existing in different forms. CD158b1 and CD158b2 (p58.2 and p58.3, respectively) are 58 kDa molecules. CD158j (p50.2) is a 50 kDa molecule. All three have identical extracellular portions, but different transmembrane and cytoplasmic regions. CD158b1 and CD158b2 likely represent allelic forms of the same locus. There is another comprehensive nomenclature which refers to these molecules as KIR2DL2 for CD158b1 (p58.2), KIR2DL3 for CD158b2 (p58.3), and KIR2DS2 for CD158j; these acronyms stand for Killer cell Immunoglobulin (Ig)-like Receptor (KIR), with extracellular part containing 2 Ig Domains (2D), and cytoplasmic tails being either long (L) or short (S). The long intracellular portion of CD158b1/b2 (p58.2/p58.3) makes them inhibitory forms, since they contain two characteristic ITIM motifs (Immunoreceptor Tyrosine-based Inhibition Motif) and are involved in the transduction of an inhibitory signal through the activation of the protein tyrosine phosphatases SHP-1 and/or SHP-2. The stimulatory form CD158h (p50.1) lacks these ITIM motifs. CD158b1/b2 (p58.2/p58.3) function as inhibitory receptors for a group of HLA-C molecules (Cw1, Cw3, Cw7 and Cw8 supertypes). The inhibitory effect is dominant over the activation exerted by the activator form p50.2. The CD158b1/b2 and CD158j molecules are indistinguishable from the extracellular viewpoint. They are expressed by a subset of NK cells, and can be detected on the surface of sub-populations of peripheral T lymphocytes, mostly of CD8+ phenotype.

Clone: GL183 Isotype: IgG1 Mouse The GL183 antibody reacts with both p58.2/p58.3 and p50.2. The antibody restores the ability of p58.2 + NK clones to lyse targets expressing HLA-C molecules.

Form

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM1846

0.2 mg

Freeze-dried

RUO

-

GMP

CD208 ( DC-LAMP ) The DC-LAMP antigen (70-90 kDa) is a new member of the LAMP (lysosome-associated membrane protein) family. As other LAMP members such as CD68, DC-LAMP is a type I integral membrane protein of 416 amino acid residues, highly glycosylated (7 N- and some O- glycosylation sites), with 90% of the molecule located in the lumen of lysosomes. DC-LAMP is specifically expressed by those mature dendritic cells (DC) located in T cell areas of lymphoid tissues, which are known as interdigitating dendritic cells (IDC). A direct correlation between the expression of DC-LAMP and that of other maturation markers such as CD86 has been established. DC-LAMP first appears in the MHC Class II compartment, suggesting an important contribution of DC-LAMP to the processing of exogenous antigens.

NIH-DC.35 cell line.

Form Isotype: IgG1 Mouse In flow cytometry, the 104.G4 antibody shows intracytoplasmic staining of DCs derived either from CD34 + cell cultures activated with TNF α and CD40L, or from monocyte cultures activated with TNF α , LPS and CD40L. Part# Format Size Status Line Quality Standard UNLB - RUO IM3448 Liquid 0.2 mg GMP Clone: 104.G4

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 188 US Research Flow Cytometry Catalog