Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD152 ( CTLA-4 ) The CD152 antigen, also called CTLA4, is an integral membrane protein belonging to the immunoglobulin superfamily, which is expressed either as a 30 kDa monomer or as a disulfide- linked homodimer. It has homology with the T cell antigen CD28, and like CD28, is a ligand for CD80 and CD86. CTLA-4 is transiently expressed by activated T cells and is not detectable in resting T cells. CD152 and CD28 are involved in the costimulation required for T cell activation. CD152 is an essential checkpoint control for autoimmunity; however, the molecular mechanism (s) by which CTLA-4 achieves its regulatory function are not well understood, especially how it functionally intersects with the CD28 pathway.

JURKAT CTLA4 Cell line.

Clone: BNI3 Isotype: IgG2a Mouse The BNI3 antibody can be used to detect intracytoplasmic CD152 (CTLA4) by flow cytometry after permeabilization with IntraPrep Permeabilization Reagent.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2282 IM2070

100 tests

Liquid

RUO RUO

IOTest

GMP GMP

UNLB

0.2 mg

Freeze-dried

-

CD157 The CD157 antigen, also known as bone marrow stromal cell antigen 1 (BST-1) is a highly glycosylated, glycosyl- phosphatidylinositol (GPI)-anchored membrane protein with a molecular weight of 43 kDa. The amino acid sequence of human BST-1 has 33% identity with CD38. Both CD38 and CD157 play dual roles as receptors and ectoenzymes, endowed with complex activities related to signaling and cell homeostasis. As with CD38, BST-1 displays ADP ribosyl cyclase activity and facilitates pre-B cell growth. CD157 is constitutively expressed by myeloid cells in peripheral blood mononuclear cells (PBMCs). The molecule is also expressed by synovial, vascular endothelial and follicular dendritic cells. Moreover, CD157 is also present on other cell types and tissues, such as dermal fibroblasts, human mast cells from lung, uterus, foreskin, and peritoneal mesothelial cells, among others.

Clone: RF3 Isotype: IgG Mouse The RF3 antibody reacts with an external epitope of BST-1 on several human cell lines including certain bone marrow stromal cell, human umbilical vein endothelial cell (HUVEC) and U937 cell lines.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM2557

0.05 mg

Liquid

RUO

-

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 187 US Research Flow Cytometry Catalog