Flow Cytometry catalog - US 2019

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Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD116 ( GM-CSFR ) The CD116 antigen, a 75-85 kDa molecule, is the human Granulocyte-Monocyte Colony- Stimulating Factor (GM-CSF) receptor α chain. The CD116 antigen is not expressed by lymphocytes, but strongly by monocytes and granulocytes as well as their precursors. It is also present on endothelial cells, fibroblasts and Langerhans cells. CD116 associates with the common β chain (CDw131) of the IL-3 and IL-5 receptors to form a high affinity receptor for GM-CSF.

Lysed normal whole blood sample.

Clone: SCO6 Isotype: IgG1 Mouse The SCO6 antibody recognizes an epitope of the GM-CSF receptor α chain. It is a high affinity antibody which binds to the membrane and to the soluble form of GM-CSFR. It immunoprecipitates the molecule. SCO6 antibody does not work in Western blot assays.

Form

Size

Format

Part#

Status

Line Quality Standard

IM1977

100 tests

Liquid

RUO

IOTest

GMP

CD120b ( TNF-R2 ) The CD120b molecule, also known as tumor necrosis factor-receptor 2 (TNF-R2) is a 75 kDa, integral membrane glycoprotein and a member of the tumor necrosis factor (TNF) receptor superfamily. Its extra-membraneous portion shows 4 cysteine-rich domains (CRDs), one of which seems to be required for ligand binding. The CD120b molecule is a receptor for TNF- α . Lymphotoxin- α (LT α ), also known as TNF- β when secreted as a soluble homotrimer, as well as the membrane-associated LT α 2/ β 1 heterotrimer. Soluble TNF resulting from cleavage of membrane TNF by the TNF- α converting enzyme (TACE) can also bind to the CD120b receptor. From a functional perspective, signaling by TNF receptor family requires clustering of 3 receptors by trimeric ligands and subsequent association of the intracellular tails with transduction factors, like the TNF receptor-associated factors (TRAF). TNF-R2 triggering can lead to NF- κ B activation, but does not result in cell death. However, the most effective cell killing occurs when membrane bound TNF crosslinks both CD120a and CD120b receptors. The CD120b molecule (TNF-R2) is restricted to hematopoietic cells. It is expressed on monocytes, neutrophils, NK cells and dendritic cells, as well as on a subset of T cells. Very weak expression can be seen on some B cells, and no expression is reported on basophils.

Lysed normal whole blood sample

Form Clone: 80M2

Isotype: IgG1 Mouse