Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD94 ( NKG2a ) CD94 is a type II transmembrane glycoprotein of 30 kDa, belonging to the Ca++-dependent (C-type) lectin family. Like NKG2A (or Kp43) on human natural killer (NK) cells, CD94 associates with one member of the NKG2 family to form disulphide-linked, NK cell receptor for MHC class I molecules, with a broader specificity than the human killer cell inhibitory (KIR) / activator (KAR) receptors of the Ig-superfamily. Ligation of CD94 either potently triggers or inhibits NK cell proliferation and cell- mediated cytotoxicity. Recent studies strongly indicate that the specificity of CD94/NKG2 receptors is for HLA-E, a non- classical MHC class I molecule. CD94/NKG2A and CD94/NKG2B heterodimers constitute inhibitory NK cell receptors, whereas the association of CD94 with NKG2C corresponds to an activating receptor. The expression of CD94 appears restricted to most NK cells and to a T lymphocyte subpopulation including a subset of γδ TCR+ T-cells and αβ TCR+ CD8+ CD56+ T-cells, mainly V α 2/V δ 2.

Clone: HP-3B1 Isotype: IgG2a Mouse Ligation of CD94 with the HP-3B1 monoclonal antibody inhibits IL-2-dependent proliferation of NK-cells, and induces apoptosis in a subset of IL-2-stimulated NK cells.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2276

100 tests

Liquid

RUO

IOTest

GMP

CD95 The CD95 (Fas or APO-1) antigen is a 40-50 kDa transmembrane glycoprotein. It belongs to the Tumor Necrosis Factor (TNF) superfamily and contains three cysteine-rich repeats. This cell surface molecule mediates apoptosis (programmed cell death). CD95 antigen is expressed on a substantial proportion of peripheral blood CD4+ lymphocytes, CD8+ lymphocytes and B lymphocytes but on a minor proportion of NK cells. It is not found on platelets or erythrocytes, but is expressed variably on granulocytes and monocytes. CD95 is strongly up-regulated on activated T cells, B cells, NK cells and thymocytes. Moreover, it is widely expressed on cell lines of T, B, NK and myeloid lineages. Most other tissues express CD95.

Clone: CH11

Isotype: IgM Mouse

Form The CH11 antibody in vitro induces apoptosis in some CD95 expressing cell lines. Part# Format Size Status

Line Quality Standard

UNLB

IM1504

0.05 mg

Liquid

RUO

-

GMP

Form Clone: UB2

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

IM1739

50 tests

Liquid

RUO

-

GMP

CD103 The CD103 antigen was originally described as the human mucosal antigen 1 (HML-1) and was subsequently proved to be the integrin α E subunit. CD103 is composed of a 150 kDa chain associated with a 25 kDa chain by a disulfide bond. It is expressed as a heterodimer in association with the integrin β 7 chain. CD103 is expressed on mucosa-associated T lymphocytes and activated cells. This human intra-epithelial lymphocyte marker is expressed in very few resting peripheral blood lymphocytes. α E β 7 binds to E-cadherin on epithelial cells. Activation and TGF β -1 induce CD103 expression.

Clone: 2G5

Isotype: IgG2a Mouse

The 2G5 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM0318

0.2 mg

Freeze-dried

RUO

-

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 184 US Research Flow Cytometry Catalog