Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD61 The CD61 antigen (platelet glycoprotein GPIIIa) is the 110 kDa integrin β 3 subunit, con-covalently associated with the integrin α IIb chain (CD41, platelet GPIIb) to form the GPIIb-IIIa complex ( α IIb- β 3 integrin). Independently of CD41, CD61 is also associated with the integrin α V (CD51) to form the vitronectin receptor. CD41/CD61 is expressed only by platelets and megakaryocytes, whereas CD51/CD61 is found on osteoclasts, endothelial cells, macrophages, fibroblasts, smooth muscle, synovial lining cells and renal glomeruli.

Clone: SZ21 Isotype: IgG1 Mouse The SZ21 antibody is specific for the PlA1, not the PlA2 form of GPIIIa. It inhibits platelet aggregation and secretion induced by collagen, arachidonic acid, and thrombin. This antibody also inhibits fibrinogen binding to human platelets induced by ADP, arachidonic acid and PAF.

Form

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM0540

0.2 mg

Freeze-dried

RUO

-

GMP

CD69 The CD69 antigen is a 60 kDa disulfide-linked dimeric structure, known as activation inducer molecule (AIM). This structure contains two differentially glycosylated forms (33 and 27 kDa) of a single core protein, that are covalently associated. This activation antigen is one of the earliest appearing cell surface glycoproteins (before other activation antigens such as IL2-R) after T or B-lymphocyte activation but is absent in resting lymphocytes. It is also expressed by activated macrophages, NK cells and other cell types including neutrophils, eosinophils and platelets. Its expression in vitro can be induced by different factors such as PMA, PHA or CD3 monoclonal antibodies.

Clone: TP1.55.3 Isotype: IgG2b Mouse The CD69 antibody is useful in combination with CD4, CD8 and CD3 antibodies for T cell activation studies. The TP1.55.3 antibody immunoprecipitates the 60 kDa homodimer and both 27 kDa and 33 kDa subunits from activated peripheral blood lymphocytes under reduced and non-reduced conditions.

Form

Size

Format

Part#

Status

Line Quality Standard

ECD

6607110

100 tests

Liquid

RUO

IOTest

GMP

CD85d ( ILT4 ) CD85d (ILT4) is a member of the new immunoglobulin (Ig)-like transcripts (ILT) family of genes located on human chromosome 19q13.4. ILT4 is also known as leucocyte Ig-like receptor (LIR)-2 and monocyte / macrophage Ig-like receptors (MIR)-10. ILT4 is a transmembrane protein recognized by 42D1 monoclonal antibody. The protein has extracellular Ig-SF domains, and ITIM motifs in the cytoplasmic tail that inhibit cellular responses by recruiting phosphatases such SHP-1. ILT4 is confined to the myelomonocytic lineage as it is expressed on monocytes, macrophages, and dendritic cells (DCs). ILT4 binds to HLA-G molecules with a 3- to 4-fold higher affinity than to classical MHC class I molecules. ILT4 may modulate one or several of the antigen presenting functions mediated by DCs, control inflammatory responses mediated by monocytes / macrophages or inhibit their cytotoxicity. The recognition of HLA-G by ILT4 may play a role in materno-fetal tolerance as HLA-G is selectively expressed in the trophoblast. It has been shown that recipient’s CD8+CD28– T suppressor (Ts) cells may induce tolerance by up-regulating ITL3 and ILT4 expressions in donor’s dendritic cells, rendering these antigen- presenting cells (APCs) tolerogenic.

Lysed normal whole blood sample.

Form Clone: 42D1

Isotype: IgG2a Rat

Size

Format

Part#

Status

Line Quality Standard

PE

A22334

100 tests

Liquid

RUO

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 183 US Research Flow Cytometry Catalog