Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD45RA CD45RA epitopes are present on all the CD45 restricted molecules in which the exon A is expressed (ABC and AB containing isoforms). Most B lymphocytes express CD45RA isoforms. The differential expression of CD45 isoforms is associated with different functional properties of T lymphocytes. Peripheral T lymphocytes can be divided into two largely distinct populations of CD45RA- CD45RO+ and CD45RA+ CD45RO - cells. CD45RA is expressed by medullar thymocytes and naïve/resting peripheral CD4+ T lymphocytes.

Form Clone: 2H4LDH11LDB9 (2H4) Size

Isotype: IgG1 Mouse

Format

Part#

Status

Line Quality Standard

FITC FITC

6603904 6603117 6603866 6603181

CYTO - STAT

50 tests

Liquid

RUO RUO RUO RUO

GMP GMP GMP GMP

Freeze-dried 100 tests

COULTER CLONE

RD1 RD1

CYTO - STAT

50 tests 100 tests

Liquid Liquid

COULTER CLONE

CD49b The CD49b antigen is a transmembrane glycoprotein of 150 kDa also known as the α -2 integrin and as platelet GPIa. CD49b non-covalently pairs with the β -1 integrin (CD29, GPIIa) to form the VLA-2 (very late activation antigen) complex. CD49b, was first identified as an extracellular matrix receptor for collagens and/or laminins. It is now recognized that the α 2 β 1 integrin serves as a receptor for many matrix and nonmatrix molecules. Extensive anlayses have defined distinct conformations that lead to inactive, partially active or highly active ligand binding. The mechanisms by which the α 2 β 1 integrin plays a critical role in platelet function and homeostasis have been carefully defined. Studies have confirmed human physiology and disease states mediated by this receptor in immunity, cancer, and development. CD49b is expressed by platelets, activated T lymphocytes and thymocytes.

A431 cell line.

Clone: Gi9 Isotype: IgG1 Mouse The Gi9 antibody reacts with the molecule carrying the Br(a) as well as the Br(b) alloantigen. This antibody inhibits the adhesion of platelets to collagen. It detects human alloantibodies (anti-Br a,b) against VLA-2 by MAIPA assay. Gi9 was used as a CD49b reference monoclonal antibody during the HLDA 6. Gi9 reacts with platelets and weakly with monocytes. It also stains most adherent cell lines and T lymphocytes in culture.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1425 IM0717

100 tests

Liquid

RUO RUO

IOTest

GMP GMP

UNLB

0.2 mg

Freeze-dried

-

CD56 ( N-CAM ) The CD56 antigen is a 140 kDa isoform of the Neural Cell Adhesion Molecule (N-CAM). Post-translational modifications to the polypeptide include N- and O- glycosylations, acylation, sulphation and phosphorylation. The different N-CAM isoforms have molecular weights ranging from 135 to 220 kDa. The CD56 antigen is moderately expressed on a subpopulation of peripheral blood large granular lymphocytes and on all cells with NK activity. It is also expressed by subsets of T lymphocytes. CD56 antibodies do not react with granulocytes, monocytes or B cells.

Form Clone: C218

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM1844

0.2 mg

Freeze-dried

RUO

-

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 182 US Research Flow Cytometry Catalog