Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD42a The CD42a antigen (GPIX) is a single-chain transmembrane glycoprotein of 22 kDa that forms a noncovalent complex with CD42b, CD42c and CD42d (GPIb α , GPIb β , and GPV). The CD42a expression is restricted to platelets and megakaryocytes. The GPIb-IX-V complex is the platelet receptor for von Willebrand factor and is known as von Willebrand's factor-dependent adhesion receptor. Although GPIb-IX-V primarily functions as a platelet receptor for von Willebrand factor (VWF), it can also bind to other ligands present in circulation such as thrombin, P-selectin, integrin α M β 2, factor XI, factor XII, high- molecular- weight kininogen, as well as a number of snake venom proteins.

Clone: SZ1 Isotype: IgG2a Mouse The SZ1 monoclonal antibody reacts with the CD42a-d complex but does not recognize GPIb or GPIX individually.

Form

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM0538

0.2 mg

Freeze-dried

RUO

-

GMP

CD42b The CD42b antigen (GPIb α ) is a membrane glycoprotein of 135 kDa, that forms a disulfide linked 160 kDa membrane protein with the CD42c (GP1b β ) known as GP1b. GP1b forms a noncovalent complex with GPIX (CD42a) and GPV (CD42d). This antigen is found on platelets and megakaryocytes. It has also been reported on vascular and tonsillar endothelial cells. The CD42 complex serves as the von Willebrand Factor(vWF) surface receptor involved in the adhesion of platelets to the subendothelium of damaged vascular walls.

Clone: SZ2 Isotype: IgG1 Mouse The SZ2 monoclonal antibody prevents the ristocetin-dependent binding of von Willebrand's factor to platelets and inhibits ristocetin-induced platelet agglutination. Platelet aggregation induced by type I collagen and Platelet Activating Factor (PAF) is also inhibited. The SZ2 antibody immunoprecipitates the components of the GPIb complex. It may also be used in Western blot.

Form

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM0409

0.2 mg

Freeze-dried

RUO

-

GMP

CD45 CD45 is a protein tyrosine phosphatase regulating src-family kinases which is expressed on all hematopoietic cells. CD45 can be expressed as one of at least five isoforms (from 180 to 220 kDa) by alternative splicing of exons that comprise the extracellular domain. The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. All the monoclonal antibodies that belong to the CD45 cluster react with this part of the antigen and are able to recognize all CD45 isoforms. The non-restricted CD45 epitope is present on the surface of all human leucocytes, lymphocytes, eosinophils monocytes, basophils and neutrophils by order of decreasing level of expression. It is absent from erythrocytes and platelets. It is lost during maturation of erythroid cells in the bone marrow. CD45 antibodies react with leucocyte progenitors in bone marrow.

Form Clone: Immu19.2

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

IM1833

100 tests

Liquid

RUO

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 181 US Research Flow Cytometry Catalog