Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD35 The CD35 antigen is a polymorphic molecule. Different molecular weights: 160, 190, 220, 250 and 285 kDa were reported for the various allotypes. It is known as the complement receptor type I (CR1) with affinity for C3b, C4b and iC3b. The CD35 antigen is expressed by granulocytes (except basophils), B lymphocytes, a subset of T-cells, monocytes, some NK cells, erythrocytes macrophages cultured in vitro, glomerular podocytes and follicular dendritic cells. The CD35 antigen mediates phagocytosis by neutrophils and monocytes of complement coated particles.

Lysed normal whole blood sample

Clone: J3D3 Isotype: IgG1 Mouse The J3D3 monoclonal antibody inhibits CR1-mediated decay of cells bound to the alternative and classical pathways to C3-convertase sites. It also inhibits C3b dependent rosette formation with lymphocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1836 IM0195

100 tests

Liquid

RUO RUO

IOTest

GMP GMP

UNLB

0.2 mg

Freeze-dried

-

CD36 The CD36 antigen (platelet GPIV, or GPIIIb) is a generic term for a family of glycoproteins with molecular weights ranging from 78 to 88 kDa. CD36 expression occurs in different types of cells, including mammary epithelial cells, endothelial cells monocytes, macrophages, platelets, megakaryocytes and early erythroid cells. This molecule is also found on some human tumor cell lines. It is a very early marker of erythroid differentiation and mediates the adherence of platelets to thrombospondin and collagen. CD36 is a scavenger receptor that functions in high affinity tissue uptake of long chain fatty acids (FA). As a result of its multiple ligands and signal transduction capabilities, CD36 has also a number of functions related to immune responses, inflammation and blood disorders.

Clone: FA6.152 Isotype: IgG1 Mouse The FA6-152 monoclonal antibody, raised against fetal erythrocytes, recognizes the CD36 family of antigens on platelets and certain hematopoietic cells. It does not react with lymphocytes or granulocytes. It reacts with both fetal and adult monocytes, megakaryocytes, platelets, and with reticulocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM0765

0.2 mg

Freeze-dried

RUO

-

GMP

CD41 The CD41 antigen (platelet GPIIb; IIb integrin) is a transmembrane glycoprotein composed of two chains GPIIb α (120 kDa) and GPIIb β (23 kDa) linked by one disulfide bond. CD41 is always non-covalently associated with CD61 (platelet GPIIIa, β 3 integrin), to form the GPIIb-IIIa (CD41/CD61) complex. CD41 is expressed by platelets, megakaryocytes and a small subset of CD34+ cells, suggesting that CD41/CD61 is one of the earliest markers of the megakaryocytic lineage. The resting form of the CD41/CD61 complex binds to immobilized fibrinogen and upon platelet activation, the complex becomes a receptor for soluble fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. It is involved in platelet aggregation.

Clone: P2 Isotype: IgG1 Mouse The P2 antibody reacts with the α chain of CD41a (GPIIb α ) in the intact complex with GPIIIa but not with the GPIIb or GPIIIa separately. This antibody blocks binding to fibrinogen and inhibits platelet aggregation induced by thrombin, collagen and ADP. The P2 antibody was used as a CD41 reference mAb during HLDA 6.

Form

Size

Format

Part#

Status

Line Quality Standard

ECD PC5 PC7

6607117 6607116 6607115 IM0145

100 tests 100 tests 100 tests

Liquid Liquid Liquid

RUO RUO RUO RUO

IOTest IOTest IOTest

GMP GMP GMP GMP

UNLB

0.2 mg

Freeze-dried

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 180 US Research Flow Cytometry Catalog