Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD25 The CD25 antigen (IL-2R α chain, Tac or p55) is a single-chain glycoprotein with a molecular weight of 55 kDa which is the low affinity receptor for IL-2 (IL-2R α or IL-2R1). In conjunction with CD122 (IL-2R β , p75) and CD132 (IL-2R γ , p64), CD25 antigen forms the high-affinity IL-2R complex. CD25 is highly expressed on regulatory CD4-positive T lymphocytes and undetected on resting CD8 positive lymphocytes. However, all activated T lymphocytes express the CD25 protein. CD25 is also found on a subset of B cells (CD20 positive), on activated monocytes and macrophages, and on T cell clones. It is also expressed on NK cells enriched from peripheral blood lymphocytes by culturing in the presence of IL-2. It is absent from thymocytes, granulocytes, monocytes, resting NK cells, platelets and erythrocytes.

Clone: B1.49.9

Isotype: IgG2a Mouse

The B1.49.9 monoclonal antibody does not block the binding of IL-2 to its receptor.

Form

Size

Format

Part#

Status

Line Quality Standard

ECD

6607112

100 tests

Liquid

RUO

IOTest

GMP

CD27 The CD27 molecule is a transmembrane disulfide-linked homodimer which belongs to tumor necrosis factor receptor (TNFR) / nerve growth factor receptor (NGFR) family. The molecular weight of the recognized antigen is 55 kDa / 120 kDa under reducing / non reducing conditions respectively. The CD27 glycoprotein is found on medullary thymocytes, peripheral T cells, subset of mature B cells and NK cells. On T cells, CD27 is preferentially expressed on the CD45RA+ CD45RO- naive subset of CD4+ T lymphocytes whereas most memory T cells (CD45RA- CD45RO+) lack CD27. Activation of T cells, when involving CD27 molecule, results in the upregulation of CD27 expression as cell surface embedded, and also in the release of a soluble form of CD27 (sCD27). CD70 antigen, which is a member of the TNF ligand superfamily, interacts with CD27 molecule and is known to be the CD27-ligand.

Form Clone: 1A4CD27

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PC5

6607107

100 tests

Liquid

RUO

IOTest

GMP

CD28 The CD28 antigen (Tp44) is a homodimer of disulfide-linked chains, with a molecular weight of 44 kDa each. It is involved in the interaction of T lymphocytes with antigen-presenting cells (APCs), through its counter-receptors, B7-1/BB-1 (CD80) and B7 -2/B70 (CD86). It provides a major co-stimulatory signal for T cell activation, proliferation and lymphokine production. The CD28 family of receptors (CD28, CTLA-4, ICOS, PD-1 and BTLA) plays a critical role in controlling the adaptive immune response. The CD28 receptor can enhance T cell antigen receptor (TCR) signals, as well as deliver independent signals. Although the signals through CD28 are crucial for the initial co-stimulation of interleukin-2 (IL-2) production, a TCR- independent CD28 signal leads to the selective transcription of survival, but not proliferative genes. CD28 is expressed by a majority of CD3+ T lymphocytes. CD4+ T lymphocytes express the CD28 antigen more frequently than do CD8+ T lymphocytes. CD28 antigen is also present on plasma cells and thymocytes.

Clone: CD28.2 Isotype: IgG1 Mouse The CD28.2 monoclonal antibody costimulates T cell proliferation and cytokine production in the presence of T cell activators ( α CD3, PMA, SEB). It inhibits proliferation of CD4 + T lymphocytes. It cross-reacts with Rhesus monkey peripheral blood lymphocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

ECD PC5

6607111 6607108

100 tests 100 tests

Liquid Liquid

RUO RUO

IOTest IOTest

GMP GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 178 US Research Flow Cytometry Catalog