Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD9 CD9 (p24 antigen) is a single transmembrane polypeptide of 24 kDa related to the tetraspanin (TM4) family. Like other tetraspanins (e.g. CD63, CD81, CD82, CD37, CD53, among the 20 known members), CD9 structure is composed of 4 transmembrane domains, with intracellular N and C termini. First discovered on a lymphoblastic cell line of pre-B phenotype, CD9 was then found on platelets and within their α -granules, on monocytes, pre-B cells, eosinophils, basophils and activated T cells. The CD9 molecule associates with other surface proteins such as the α 6/ β 4 integrin (CD49f/CD104 molecule) and HLA- DR, suggesting a role in adhesion, signal transduction and cell motility.

Clone: ALB6 Isotype: IgG1 Mouse The ALB6 monoclonal antibody (mAb) was first reported as specific for CD9 (p24) on platelets, capable of modulating aggregation of platelets activated with various agonists.

Form

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM0117

0.2 mg

Freeze-dried

RUO

-

GMP

CD16 The CD16 antigen is the low-affinity receptor for IgG (Fc γ RIII). The CD16 antigen exists in two different forms encoded by two different genes: Fc γ RIIIA (or III-2) and Fc γ RIIIB (or III-1). The genetic heterogeneity of CD16 generates alternative membrane- anchored molecules. One is a transmembrane form (Fc γ RIIIA, 50-65 kDa) expressed on NK cells, monocytes and macrophages. The other is a glycosylphosphatidylinositol (GPI)-anchored form (Fc γ RIIIB, 48 kDa) only expressed on neutrophils. It has been shown that the CD16 antigen can be non-covalently associated within the membrane of NK cells, to a disulfide-linked homo or heterodimer made from the 16 kDa CD3 ζ chain and the Fc ε RI γ chain.

Clone: 3G8 Isotype: IgG1 Mouse The 3G8 monoclonal antibody is inappropriate for western blot use. 3G8 binds to both isoforms of the CD16 molecule.

Form

Size

Format

Part#

Status

Line Quality Standard

PC7

6607118

100 tests

Liquid

RUO

IOTest

GMP

CD24 The CD24 antigen is a glycosyl-phosphatidylinositol-linked membrane sialoglycoprotein, with a molecular weight of 35-45 kDa. CD24 is present on B cells, from the pre-B to the mature B cell stage, but not on plasma cells. It is expressed on mature granulocytes and on a variety of epithelial cell types. CD24 was discovered in mice as a heat-stable antigen and was used as a marker to differentiate hematopoietic cells and neuronal cells. It is currently known that CD24 serves as a costimulatory factor of T cells that regulate their homeostasis and proliferation, while in B cells, CD24 is functionally involved in cell activation and differentiation. In hematopoietic cells, CD24 is known to bind to molecules exhibiting danger-associated molecular patterns (DAMPs). CD24 mediates signal transduction and activates the mitogen-activated protein kinase pathway, which involves B- and T-cell development and apoptosis, cell binding and granulocyte oxidative burst.

Form Clone: ALB9

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

UNLB

IM0118

0.2 mg

Freeze-dried

RUO

-

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 177 US Research Flow Cytometry Catalog