Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

CD4 The CD4 antigen is a monomeric transmembrane glycoprotein with a molecular weight of 59 kDa. The intracytoplasmic tail of CD4 is essential for interaction with Lck. The CD4 is expressed on the T "helper" (Th or T4) lymphocytes. It is expressed on most thymocytes, where it is frequently co-expressed with CD8. CD4 is also expressed on eosinophils. All the monocytes carry the CD4 antigen, but at a lower density than T4 lymphocytes. CD4 acts as an accessory molecule to the T cell receptor complex during T-cell activation restricted to the major histocompatibility complex class II. The T4 lymphocytes are involved in T-T, T-B, and B-macrophage cellular interactions. They are active in inducing and helping the synthesis of immunoglobulins by B cells. CD4 functions as the receptor for IL-16 which is a chemoattractant factor for CD4+ T cells, as well as for monocytes and eosinophils. IL-16 seems to be a growth factor for T4 lymphocytes, but is not able to induce cell division. CD4 is a receptor for the Human Immunodeficiency Virus type I (HIV-1) envelope protein gp120.

Clone: SFCI12T4D11 (T4)

Isotype: IgG1 Mouse

Form SFCI12T4D11 monoclonal antibody antagonizes HIV effects in vitro . Part# Format Size

Status

Line Quality Standard

PC7

6607101

100 tests

Liquid

RUO

IOTest

GMP

CD8 The CD8 antigen is a disulfide-linked dimer, which exists either as a CD8 α homodimer or as a CD8 α / β heterodimer. CD8 α is required for surface expression of CD8 β . The molecular weight of each monomer of α or β is approximately 32-34 kDa. CD8 binds to a non-polymorphic domain ( α 3 domain) of MHC Class I molecules. CD8 is expressed on a subset of human peripheral blood T lymphocytes. A subset of NK cells possess the CD8 antigen but show low to medium density of expression. CD8 α homodimer is expressed by NK cells and γ / δ + T cells. CD8 is also present on most thymocytes where it is frequently co- expressed with CD4, and on a subpopulation of bone marrow cells. The CD8 molecule acts with the T Cell Receptor (TCR) as a coreceptor for MHC class I restricted antigen recognition. CD8 is widely used as a marker of cytotoxic T lymphocytes.

Clone: SFCI21Thy2D3 (T8)

Isotype: IgG1 Mouse

Form SFCI21Thy2D3 monoclonal antibody recognizes the α chain of the CD8 molecule. Part# Format Size Status

Line Quality Standard

PC7

6607102

100 tests

Liquid

RUO

IOTest

GMP

CD8 β Along with CD8 α , the CD8 β polypeptide molecule is one of the two chains which can be expressed within the CD8 dimer. CD8 α is required for surface expression of CD8 β . The α and β glycoproteins have similar molecular weights of 32-34 kDa each. They form homodimeric (CD8 α / α ) or heterodimeric (CD8 α / β ) cell surface complexes. Most of human peripheral T cells express CD8 as an α / β heterodimer. NK cells and a proportion of TCR γ / δ T cells express CD8 as an α / α homodimer. CD8 β surface expression requires the surface expression of CD8 α . Consequently, CD8 α can form homodimer but CD8 β cannot.

Form Isotype: IgG2a Mouse The 2ST8.5H7 monoclonal antibody reacts with an epitope formed by the combination of the α and β polypeptides. Part# Format Size Status Line Quality Standard ECD IOTest RUO 6607123 Liquid 100 tests GMP UNLB - RUO IM2014 Freeze-dried 0.2 mg GMP Clone: 2ST8.5H7

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 176 US Research Flow Cytometry Catalog