Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Research Use Only (RUO) Reagents

β 2-microglobulin The β 2-microglobulin is the non-polymorphic non-covalently bound light chain (12 kDa) of the class I major histocompatibility complex (MHC-I) constitutively expressed on the surface of almost all nucleated cell. β 2-microglobulin structure, stabilized by a disulfide bound, is similar to an immunoglobulin like domain. β 2-microglobulin is required in the endoplasmic reticulum (ER) to associate with the MHC class I heavy molecule to form with other proteins (calreticulin, tapasin, etc.) the loading complex that exits the ER and is transported to the cell surface. After peptide presentation, β 2-microglobulin is actively released into circulation.

Lysed normal whole blood sample

Form Isotype: IgG2a Mouse The B1G6 monoclonal antibody is specific for human β 2-microglobulin. It does not react with β 2-microglobulin from other species. It recognizes the β 2-microglobulin, free or associated with human HLA class I molecules. Part# Format Size Status Line Quality Standard UNLB - RUO IM0114 Freeze-dried 0.2 mg GMP CD1a Clone: B1G6

CD1a is a member of the lipid antigen presenting molecules family Like the other CD1 proteins (group 1: CD1a, CD1b, CD1c and group 2: CD1d, CD1e), CD1a is a transmembrane protein of 49 kDa, noncovalently associated with β 2-microglobulin. As other group 1 CD1 antigens, CD1a is found on professional antigen-presenting cells (APC) such as dendritic cells, particularly on Langerhans cells and their direct circulating precursors. It is expressed by those cells related to dermal dendritic cells, derived from monocytes cultured for 6 days in the presence of GMCSF and IL-4. The CD1a antigen is also present on a B cell subset. It is also strongly expressed by cortical thymocytes. CD1a antibodies are useful in the definition of the thymic stage of T cells.

Clone: SFCI19Thy1A8 (T6)

Isotype: IgG1 Mouse

T6 (ref 6T-CD1.7) was used as a CD1a reference monoclonal antibody during HLDA 6.

Form

Size

Format

Part#

Status

Line Quality Standard

RD1

6603185

100 tests

Liquid

RUO

GMP

COULTER CLONE

CD3 CD3 ζ see TCR ζ .

The CD3 antigen is a complex of 5 invariable polypeptidic chains: γ , δ , ε , ζ and η , whose molecular weights are respectively 25 -28, 21, 20, 16 and 22 kDa. The CD3 chains are clustered in a group of two invariant dimers, γ / ε and δ / ε associated with a variable dimer which consists of ζ homodimers, or ζ / η , or ζ / γ FcR heterodimers ( γ FcR being the γ chain of the Fc receptors), or γ FcR homodimers. The CD3 is part of a bigger complex which includes the T Cell Receptor (TCR). CD3 complex associated with the TCR is involved in the recognition of peptides bound to the major histocompatibility complex class I and II during the immune response. T cell activation may be induced when a foreign antigen is presented to the TCR through MHC complex. The CD3 antigen is expressed by mature T lymphocytes and by a subset of thymocytes.

Clone: UCHT1 Isotype: IgG1 Mouse UCHT1 reacts with the ε -chain of the CD3 complex. It was used as a CD3 reference mAb (ref.6T-CD3.1) during HLDA 6. After permeabilization with saponin-based permeabilization reagent IntraPrep, it may be used to detect intracytoplasmic CD3 by flow cytometry.

Form

Size

Format

Part#

Status

Line Quality Standard

PC7

6607100

100 tests

Liquid

RUO

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 175 US Research Flow Cytometry Catalog