Flow Cytometry catalog - US 2019

Lysing, Fixative and Permeabilizing Solutions

For Intra- and Extra- Cellular Staining Preparation PerFix-nc ( no centrifuge assay kit )

The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation. Accurate detection of both intracellular and extracellular epitopes are obtained, while: • There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as optional). • Several surface markers can be added together with the intracellular markers and incubated simultaneously during the permeabilization step. • Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g. VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures. • Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the washing steps.

Size

Status

Quality Standard

Part #

Fixative Buffer : 1 vial Permeabilizing Buffer : 1 vial Final 10X Solution : 1 vial Fixative Buffer : 2 vials Permeabilizing Buffer : 2 vials Final 10X Solution : 2 vials

75 tests

IVD & CE

GMP

B31167

150 tests

IVD & CE

GMP

B31168

Illustration of leucocyte pattern and intracytoplasmic B-Cell staining on normal sample, implementing the PerFix-nc STRAIGHT protocol.

ZAP-70-PE staining on lymphocytes of another normal sample, implementing the PerFix- nc + wash protocol.

SUPPORT REAGENTS

162

US Clinical Diagnostics Catalog