Flow Cytometry catalog - US 2019

Lysing, Fixative and Permeabilizing Solutions

Beckman Coulter provides a complete comprehensive workflow solution for all your flow cytometry needs. Product Principle Sample Fixation in Wash / No-wash Fixation Applications lysis step after lysis

Lysis without permeabilization

Versalyse

Ezyme-based erythrocytes lysis Venous blood,

Optional

Can be used both

Yes

Extracellular staining, Low stringency, Gentle to cells

bone marrow (IOTest3 Fixative washed and

Solution)

no-wash

IOTest 3

Ammonium Chloride based

Venous blood, bone Marrow

No

Wash after lysis

Optional

Classical Reference Method

Lysing Solution erythrocyte lysis

(IOTest3 for extracellular staining Fixative)

OptiLyse C

Formaldehyde-glycerol -based erythrocytes lysis.

Venous blood, bone marrow

Yes

Designed for no-wash

Yes

Extracellular staining on Beckman Coulter instruments

Whole Blood

Amphipathic glycoside

Venous blood

No

Wash

Yes

Extracellular staining. Optimized for use with COULTER CLONE and CYTO - STAT COULTER CLONE antibodies Extracellular staining. Automatisation only Used on Q-PREP, FP1000, Multi-Q-prep and TQ-prep

Lysing Reagents based lysis

IMMUNOPREP

Formic acid-based lysis targeted for immunofluorescence measurements on flow cytometers

Venous blood

Yes

No-wash

Yes

Lysis with permeabilization

IntraPrep

Formaldehyde/saponin-

Venous blood, bone marrow

Yes

Wash

Yes

Classical Reference Method for intracellular staining

Permeabilization based Permeabilization

Reagent

PerFix-nc

Low Formaldehyde/Low detergent Venous blood,

Yes

Optional

Yes

Allows simultaneous staining of both extracellular and intracellular markers No wash procedure providing high resolution. High Stringency reagent. Phospho epitope detection. Allows the simultaneous staining of both extracellular markers, intracellular markers and phospho epitopes.

-based permeabilisation.

peripheral blood mononuclear cells, mouse blood, bone marrow

PerFix EXPOSE Detection of phospho epitopes

Venous blood,

Yes

Wash

Yes

by fixation, erythrocyte lysis, and bone marrow,

permeabilization.

peripheral blood mononuclear cells

Fixation

IOTest 3 Fixative Solution

Formaldehyde-based fixative, provided as 10X

Venous blood, bone marrow

Yes

Not Applicable

Yes

Optimized to work in combination with IOTest 3 Lysing Solution, VersaLyse Lysing Solution and other systems.

concentrate.

SUPPORT REAGENTS

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US Clinical Diagnostics Catalog