Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

TIA-1 The T cell intracellular antigen 1 (TIA-1) is a 17 kDa cytoplasmic granule-associated protein, expressed in cells possessing cytolytic potential. TIA-1 is also designated as GMP-17 (granule membrane protein of 17 kDa). The GMP-17/TIA-1 molecule could be involved in the molecular signaling cascade during Fas (CD95)-mediated apoptosis. In normal peripheral blood lymphocytes, TIA-1 is expressed by 90% of CD16+, 50-60% of CD8+, and less than 10% of CD4+ cells. Although TIA-1 may be detected in unstimulated T lymphocytes, activated T lymphocytes are induced to express disulfide linked polymers of the 17 kDa protein, also recognized by TIA-1. TIA-1 is expressed by all monocytes and granulocytes, by activated CD4+ T-cell clones and activated natural killer cell clones, and by Con A-activated thymocytes. On the contrary, TIA-1 is not expressed by B lymphocytes or B-cell lines.

Lysed normal whole blood sample.

Clone: 2G9A10F5 Isotype: IgG1 Mouse The 2G9 antibody is a useful reagent for detecting cytolytic effector cells in tissue infiltrates. It was evaluated during HLDA 5 in the section of monoclonal antibodies reactive with intracellular antigens. It may be used to detect intracytoplasmic TIA-1 in flow cytometry after permeabilization with IntraPrep permeabilization reagent.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM3293 IM2550

2 mL

Liquid

ASR ASR

IOTest

GMP GMP

UNLB

0.2 mg

Freeze-dried

-

TNF α Tumor necrosis factor α (TNF α ) or cachectin is a cytokine that was first identified based on its cytotoxic activity against various cell lines and tumor cells. Human TNF α is synthesized as a pro-protein with a molecular weight of 26 kDa (membrane-bound form). The pro-protein is cleaved by a specific metalloprotease (TACE) yielding a monomeric, soluble form of 17 kDa. Under physiological conditions, TNF α forms a homotrimeric protein. This cytokine is primarily produced by mononuclear phagocytes, and by activated lymphocytes. Immunostaining allows intracellular detection of TNF α before it can be detected by a bioassay. TNF α acts on target cells by binding to two types of receptors, the TNF α receptor I (TNF-RI or CD120a) and the TNF α receptor II (TNF-RII or CD120b). TNF α elicits a wide spectrum of immune and inflammatory responses, including the induction of other cytokines and immunoregulatory molecules, cell growth and differentiation and apoptosis. This molecule is also involved in Th1 - Th2 (T helper 1 - T helper 2) cytokine pathways (or Type 1 - Type 2 responses), as a Th1- effector cytokine.

Activated PBL.

Form Clone: IPM2 (188)

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

IM3279U B76295

2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

AF700

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 133 US Clinical Diagnostics Catalog