Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

TdT Terminal deoxynucleotidyl transferase (TdT) is a nuclear enzyme with a molecular weight of 58 kDa. This molecule catalyses random addition of nucleotides on the 3’ OH termini of single-stranded DNA. TdT differs from other DNA polymerases because it does not require a template sequence for polymerization. TdT is mainly expressed during the maturation of the immune system in primary and secondary lymphoid organ cells: i.e. precursors of B lymphocytes in the bone marrow and precursors of T-lymphocytes in the thymus. The TdT expression is not found in myeloid, erythroid, or megakaryocytic precursors, or in other non lymphoid cell components in every tissue, including mesenchymal, epithelial, and germ cells.

Form Clone: HT1 + HT4 + HT8 + HT9 Size

Isotype: IgG Mouse

Format

Part#

Status

Line Quality Standard

FITC

IM3524U

1 mL

Liquid

ASR

-

GMP

Thrombin receptor Thrombin is a coagulation protease produced at sites of vascular injury that activates platelets, endothelial cells, leukocytes and mesenchymal cells via cleavage of specific cell surface receptors known as proteinase activated receptors (PARs). A functional thrombin receptor from human platelets has been cloned and sequenced. It is a 66-kDa, single polypeptide chain that belongs to the cell surface G-protein-coupled receptor family, with seven transmembrane domains and an extracellular N terminus. The thrombin cleavage site is located in the N terminus between Arg41 and Ser42. Following cleavage by thrombin, activated receptors undergo desensitization and internalization but a fraction of them are recycled to the cell surface. The Thrombin Receptor is expressed on a variety of cells including platelets and endothelium. The receptor is a substrate of thrombin and has been shown to play a role in the activation of platelets. Isotype: IgG1 Mouse The SPAN 12 antibody recognizes an epitope on the receptor that spans the thrombin cleavage site. It binds to intact receptors, but not to cleaved ones. SPAN 12 inhibits thrombin-induced receptor activation at reduced thrombin concentrations. Clone: SPAN 12

HEL Cell line.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2583

2 mL

Liquid

ASR

IOTest

GMP

Clone: WEDE15 Isotype: IgG1 Mouse The WEDE15 antibody recognizes an epitope on the receptor, distant from the N-terminal thrombin cleavage site (WEDEE). It reacts with both intact and cleaved receptors.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2584

2 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 132 US Clinical Diagnostics Catalog