Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

TCR V γ 9 V γ 9 is the only functional member of the group II γ variable genes of the γδ T cell receptor. It is expressed by 0.5% to 10% of PBL in normal blood, representing around 70% of circulating γδ + T cells. Therefore, V γ 9 positive cells are the major population of circulating γδ T cells. V γ 9 is mainly associated with V δ 2. The combination V γ 9-V δ 2 γδ T cell seems to recognize mycobacterial antigens. This sequence is also referred to as TRGV9 (based on the IMGT gene nomenclature).

Lysed normal whole blood sample gated on lymphocytes.

Clone: IMMU 360 Isotype: IgG1 Mouse IMMU 360 may be used in flow cytometry, immunoprecipitation, Western blot and immunohistochemistry techniques.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC PC5

IM1463 A63663

1 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

0.5 mL

TCR ζ The ζ subunit is a 16 kDa surface molecule associated with the T cell receptor. Its expression is restricted to T lymphocytes and NK cells. In T lymphocytes, the ζ subunit is expressed as a disulfide-linked homodimer of 32 kDa or as a disulfide-linked heterodimer with a 21 kDa subunit designated η . Both of these dimeric species are non-covalently associated with the remaining subunits of the CD3-TCR-antigen receptor complex. In NK cells, the ζ subunit is expressed as a disulfide-linked homodimer of 32 kDa or as a disulfide-linked heterodimer with a 12 kDa subunit. It is associated with the CD16 molecule (Fc γ RIII).

JURKAT Cell line.

Form Isotype: IgG1 Mouse The 2H2D9 (TIA-2) antibody recognizes a cytoplasmic epitope of the ζ subunit of the T lymphocyte receptor complex. It is suitable for immunoprecipitation, immunoblotting, and flow cytometric analysis of permeabilized cells. The TIA-2 antibody was evaluated during the Fifth International Workshop on Human Leukocyte Differentiation Antigens. TIA-2 antibody can be used to detect intracytoplasmic TCR- ζ in flow cytometry after permeabilization with digitonin. Part# Format Size Status Line Quality Standard PE IOTest ASR IM3169 Liquid 2 mL GMP Clone: 2H2D9 (TIA-2)

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 131 US Clinical Diagnostics Catalog