Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

TCR PAN γ / δ The TCR is a molecular complexe wich comprises two units: a recognition unit, composed of either alpha-beta or gamma-delta heterodimer, which are present on the cell surface in a mutually exclusive manner, and, a transducing unit, the CD3 complex, common to alpha-beta and gamma-delta heterodimers, which triggers the T cell when the recognition unit is occupied by the antigen. The recognition unit recognizes foreign antigens and the diversity necessary for this function of recognition is generated by somatic recombination the TCR genes. There are four TCR gene loci (alpha, beta, gamma and delta). Each of them is composed of several V (variable) segments, coding for about 90 amino acids, very short D (diversity) segments (alpha and delta loci only), and short J (joining) segments (about 15 amino acids), and one or two C (constant) segments. Most of T cells express the alpha/beta TCR (T cell receptor) protein and a small population of T cells expresses the gamma/delta TCR, which usually has a double negative (CD4/CD8) phenotype. Gamma/delta T-cells are normally the first line of defence at epidermal and epithelial surfaces and they represent 10-12% of lymphocytes in the spleen. Isotype: IgG1 Mouse The IMMU510 monoclonal antibody recognizes all γ / δ T cells, regardless of their variable chains. It stains 1.6% to 8.9% of peripheral CD3 + cells from healthy donors. In Western blot, it recognizes the δ chain in reducing conditions. It immunoprecipitates the three isoforms of the γ / δ T cell receptor. This antibody is suitable for immunohistochemistry on frozen sections. It has been used to study the distribution of γ / δ T cells and their functions. Part# Format Size Status Line Quality Standard FITC IOTest ASR IM1571U Liquid 1 mL GMP PE IOTest ASR IM1418U Liquid 1 mL GMP PC5 IOTest ASR IM2662U Liquid 0.5 mL GMP PC5.5 IOTest ASR A99021 Liquid 0.5 mL GMP PC7 IOTest ASR B10247 Liquid 0.5 mL GMP TCR V α 24 Also called TCRAV24S1 (according to the nomenclature from Arden et al.) V α 24 is the only described member of the subfamily of the T cell receptor. It has been shown to be expanded in a clonal fashion in the CD3+ CD8- CD4- cell population in PBL of several donors. It is expressed by 0.4% to 1% of peripheral CD3+ cells from normal blood. An invariant V α 24/V β 11 T cell receptor is expressed in all individuals by clonally expanded CD4– CD8– T cells, reactive to bacterial antigens. This unique lymphocyte population restricted by the CD1d molecule recognition has been identified as the natural killer T (NKT) cells. The IGRa02 sequence is also referred to as TRAV10 (based on the IMGT gene nomenclature). Clone: IMMU510

Lysed normal whole blood sample.

Form

Tri VAlpha24 Cell line.

Clone: C15

Isotype: IgG1 Mouse

The C15 antibody recognizes the IGRa02 sequence, the only described member of this family.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1589 IM2283 A66907

1 mL 1 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

GMP GMP GMP

PE

PC7

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 117 US Clinical Diagnostics Catalog