Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

Myeloperoxidase Myeloperoxidase (MPO) is an heterodimeric glycoprotein of 150 kDa with an α 2/ β 2 structure. The two subunits ( α and β ) have a molecular weight of 55 and 15 kDa, respectively. MPO synthesis occurs in bone marrow at an early stage of myeloid lineage differentiation. MPO is specifically expressed during promyelocytic formation, the stage at which azurophilic granules (or primary granules) are formed. MPO is still found in mature myeloid cells, becoming the major constituent of azurophilic granules of neutrophils. MPO is stored in polymorphonuclear neutrophilic granules and in macrophages, but it is not expressed in lymphocytes, platelets and erythrocytes. MPO catalyzes the formation of hypochlorous acid (HOCl) in the presence of active oxygen (H2O2). Intranuclear MPO may also help to protect DNA against damage resulting from oxygen radicals produced during cell maturation and function.

Permeabilized normal whole blood sample.

Clone: CLB-MPO-1 Isotype: IgG2a Mouse The CLB-MPO-1 antibody recognizes MPO and its enzymatically inactive form. This antibody can be used to detect intracytoplasmic myeloperoxidase in flow cytometry after permeabilization with IntraPrep permeabilization reagent.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1874U IM3455U

2 mL 2 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

GMP GMP GMP

PE

PC5.5

B23132

0.5 mL

NG2 ( 7.1 ) Human melanoma-associated chondroitin suflate proteoglycan (MCSP), known as the homolog rat NG2 antigen, is a 250 kDa glycoprotein that is though to play important roles in angiogenesis-dependent processes like wound healing and tumor growth. The core protein contains an open frame of 2322 amino acids encompassing a large extracellular domain, a hydrophobic transmembrane region and a relatively short cytoplasmic tail. This antigen is not expressed on normal hematopoietic cells but can be expressed on myeloid and lymphoid cell subsets with chromosomal modifications. It is also expressed on human glial cells. This antigen regulates signaling events that are important for both cell proliferation and cell migration through at least two distinct mechanisms: the focal adhesion kinase (FAK) and the extracellular signal-regulated kinases (ERK) 1/2.

HELA cell line.

Clone: 7.1 Isotype: IgG1 Mouse The 7.1 monoclonal antibody recognizes the human melanoma associated chondroitin sulfate proteoglycan molecule.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM3454U

2 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 114 US Clinical Diagnostics Catalog