Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

κ Chain Kappa ( κ ) chain is a 25Kd polypeptide that results from papain cleavage of the IgG molecule, encoded by the human immunoglobulin kappa locus (IGK) on chromosome 2. The subunit structure of IgG comprises 2 light chains and 2 heavy chains. Each light chain is composed of two tandem immunoglobulin domains: a) one variable domain (VL) and b) one constant (CL) domain. While the VL show considerable sequence diversity the CL shows internal homology with repeating units. Anti-Kappa antibodies recognize the Kappa light chain of immunoglobulins expressed at the surface of a cellular subpopulation corresponding to approximately 2/3 of mature B lymphocytes in peripheral blood. This light chain is also found on the surface of a sub-population of immature bone marrow B lymphocytes.

Lysed normal whole blood sample

Clone: Polyclonal

Isotype: F(ab’) 2

Goat

Form

Size

Format

Part#

Status

Line Quality Standard

FITC APC

C15623 B90420

2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

0.5 mL

NEW

Clone: Polyclonal

Isotype: F(ab’) 2

Rabbit

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

A64828

2 mL

Liquid

ASR

IOTest

GMP

Ki-67 Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is never expressed by resting (G0) cells. The Ki-67 antigen is a human nuclear protein defined by its reactivity with monoclonal antibody from the Ki-67 clone. Two isoforms of 345 and 395 kDa have been identified by cDNA sequence coding. Deduced amino acid sequence analysis of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences. The Ki-67 antigen is expressed at active phases of the cell cycle (G1, S, G2 and M phases), but it is absent in resting cells (G0 phase). The level of Ki-67 antigen expression varies during cell cycle and it has been correlated to several cell pathways: The Ki-67 decrease pathway is characterized by a declining Ki-67 staining and leads eventually to the exit from the active cell cycle (G0). If cells on this pathway get stimulated by growth factors, they can enter the Ki-67 increase pathway that brings the cells back into S phase. Cells following the Ki-67 stable pathway exhibit a constant intensity of Ki-67 staining during the G1 phase. This pathway is thought to correspond to optimal local growth conditions. The cellular localization of the Ki-67 protein is cell cycle phase dependent. During interphase, the antigen can be exclusively detected within the nucleus, whereas in M phase most of the protein is relocated to the surface of the chromosomes. The antigen is rapidly degraded as the cell enters the non proliferative state.

Lysed normal whole blood sample spiked with MOLT4 cell line

Form Clone: Ki-67

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

AF488

B68180

0.5 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 112 US Clinical Diagnostics Catalog