Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

HLA-ABC HLA-A, -B, and -C are major histocompatibility complex (MHC)-class I antigens. Like other class I molecules (i.e. HLA-E, -F, -G), HLA-A, -B, and -C are heterodimers consisting of a 40 – 45 kDa transmembrane glycoproteic α -chain, noncovalently combined to the invariant β 2- microglobulin. All class I molecules have conserved, monomorphic domains, but are also characterized by their extensive degree of allelic polymorphism. MHC molecules play a central role in the immune response. They are involved in the maturation of T cell repertoire, in the activation of T lymphocytes by presentation of xenogenic peptides or in the allogenic response. HLA-A, -B and -C are “classical” MHC Class I molecules and are expressed on the surface of most nucleated human cell types.

Lysed normal whole blood sample.

Clone: B9.12.1 Isotype: IgG2a Mouse The B9.12.1 monoclonal antibody recognizes a monomorphic epitope common to HLA-A, -B and -C molecules. B9.12.1 antibody does not recognize unassociated heavy chains.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1838U

2 mL

Liquid

ASR

IOTest

GMP

HLA-DR The human major histocompatibility complex (MHC), also called human leucocyte antigens (HLA), is composed of three groups of molecules designated MHC class I, class II and class III. The MHC class II genomic region, or HLA-D region, contains the genes encoding HLA-DR, -DQ and -DP antigens. The MHC class II molecules are built by the non-covalent association of α / β heterodimers. Both heavy ( α ) and light ( β ) chains span the cell membrane. They have molecular weights of 31-33 kDa and 26-29 kDa respectively. HLA-DR molecules are found on antigen-presenting cells (APC), i.e. dendritic cells, B lymphocytes, monocytes, macrophages, Langerhans cells and thymic epithelial cells. They are also expressed on activated T cells, but not on granulocytes, platelets, or red blood cells. Resting T cells or endothelial cells that do not normally express these molecules can be induced to express them. HLA-DR is also expressed on some hematopoietic progenitor cells at different stages of differentiation. Isotype: IgG2b Mouse HLA-DR antibodies are specific to non polymorphic determinants of human HLA class II DR molecules which are constituted of α and β polypeptides of 34 and 29 kDa respectively. Part# Format Size Status Line Quality Standard FITC IOTest ASR IM0463U Liquid 2 mL GMP PE IOTest ASR IM0464U Liquid 2 mL GMP Form Clone: B8.12.2

Lysed normal whole blood sample.

Form Clone: Immu-357

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PB

A74781 B00070 IM1638U IM1639U IM3636 IM2659U B20024 A40579 IM3635 B42021

0.5 mL 0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR ASR ASR ASR ASR ASR ASR ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

GMP GMP GMP GMP GMP GMP GMP GMP GMP GMP

KrO FITC

2 mL 2 mL 1 mL 1 mL

PE

ECD PC5

PC5.5

0.5 mL 0.5 mL

PC7 APC

1 mL

APC-A750

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 109 US Clinical Diagnostics Catalog