Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

FoxP3 FoxP3 (Forkhead box protein P3, also known as Scurfin, JM2, or IPEX), is the major transcription factor that determines the fate and identity of CD4+ CD25+ Treg cells. It is constitutively expressed in natural Tregs (nTregs) and acts as the master regulator in nTreg development and suppressive function. nTreg (CD4+ CD25+ FoxP3+ Treg cells) are important regulators of almost all immune responses, and they mediate suppressive functions both through the production of cytokines and direct cell–cell contacts. nTreg cells exit in the thymus, circulate and migrate to secondary lymphoid tissues, as well as to inflammatory sites, to exert suppressor activities. FoxP3 expression can also be directly induced in peripheral naïve CD4+ CD25– T cells, forming inducible Treg (iTreg) cells that also suppress immune responses. Diminished Treg cell development and function has been linked to auto-immunity and allergy whereas elevated Treg cell reponses might play a role in undermining anti-infectious and anti- cancer immunity. FoxP3 contains 3 discernible functional domains, a single C2H2 zinc-finger motif, a leucinezipper- like motif, and a C-terminal forkhead domain, thereby exerting its positive and negative function as transcription factor. FoxP3 acts as transcriptional activator for genes that are typically up-regulated in nTregs while it represses transcription of both Th1 and Th2 cytokines in nTregs. FoxP3 can also exert regulatory capacity via protein-protein interactions.

Permeabilized normal whole blood sample. Gating on lymphocytes. Dual staining with CD4-FITC and FoxP3- A647.

Clone: 259D

Isotype: IgG1 Mouse

The monoclonal antibody 259D detects endogenous levels of human FoxP3 transcription factor.

Form

Size

Format

Part#

Status

Line Quality Standard

PB PE

B90432 B46031 B46032 B30650

0.5 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

1 mL

PC7

0.5 mL 0.5 mL

AF647

Granzyme B Granzyme B (GrB) is a single chain and single domain serine protease. It is a member of the chymotrypsin superfamily. The process of GrB glycosylation results in generation of a 32 kDa and a 35 kDa glycosylated forms of GrB. The 32 kDa GrB forms contain high mannose oligosaccharide moieties and accumulate in cytotoxic T lymphocytes (CTLs) after T cell receptor (TCR) stimulation. In contrast, the 35 kDa GrB forms, which possess only the complex oligosaccharide groups, are not stored in CTLs and instead they are secreted through the constitutive calcium-independent secretory pathway after TCR activation. GrB is the most abundant serine protease stored in secretory granules of CTLs and NK cells. GrB can be produced by plasmacytoid dendritic cells (pecs). GrB-induced cell death is a primary mechanism in cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells to eliminate harmful target cells including allogeneic, virally infected and tumor cells. This mechanism implies activation of several pro-apoptotic pathways by direct proteolysis. The mannose 6-phosphate receptor has been identified as the plasma membrane receptor for GrB.

Lysed normal whole blood sample.

Clone: GB11

Isotype: IgG1 Mouse

The monoclonal antibody GB11 recognizes the human, mouse and rat Granzyme B.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

B46038

1 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 108 US Clinical Diagnostics Catalog