Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

Cytokeratin Cytokeratins belong to the intermediate filament protein family and are characteristic of epithelial cells. At present, more than 20 different cytokeratins have been identified based on the original two dimensional gel electrophoresis classification. A sub classification, based on charge, divides cytokeratins into two subfamilies: the acidic (negatively charged) cytokeratin (type 1) and the neutral to basic (positively charged) cytokeratin (type II). They are typically expressed as pairs, consisting of one acidic and one basic molecule.

HELA Cell line.

Clone: J1B3 Isotype: IgG1 Mouse This monoclonal antibody exclusively reacts with cells of epithelial origin, and shows a cytoplasmic staining pattern. It can be used as a gating tool in flow cytometry to distinguish epithelial from non-epithelial cells. Multi-color flow cytometric analysis requires preliminary permeabilization of the cells with digitonin or methanol.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM2356U

2 mL

Liquid

ASR

IOTest

GMP

FMC7 ( CD20 ) FMC7 is a transmembrane glycoprotein with a molecular weight of 105 kDa. This antigen was described as a particular conformation of a surface protein encoded by the CD20 gene. FMC7 antibody reactivity to its particular CD20 epitope was found to be dependent of membrane cholesterol level. In circulating blood cells, FMC7 is expressed by a B lymphocyte sub- population. It is not detected on T lymphocytes, NK cells, polymorphonuclear granulocytes, platelets, nor on erythrocytes. The subpopulation of B lymphocytes expressing the FMC7 antgen expresses surface immunoglobulins, thus defining a stage of maturation of the B lymphocytes distinct from FMC7– B lymphocytes.

Clone: FMC7 Isotype: IgM Mouse The FMC7 antibody reacts with a subpopulation of peripheral blood B lymphocytes and tonsil B cells, but it does not react with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells.

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B08466 IM1364U

0.5 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

FITC

1 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 107 US Clinical Diagnostics Catalog