Flow Cytometry catalog - US 2019

Single-Color Conjugated Antibodies

Analyte Specific Reagents - ASR product list

CD336 ( NKp44 ) CD336, also known as NKp44, is a 44-kDa transmembrane glycoprotein which is selectively expressed by activated NK cells. NKP44 functions as a triggering receptor involved in non- MHC-restricted natural cytotoxicity by activated NK cells and is one of the NK natural cytotoxicity receptors (NCRs). Structurally, CD336 is a member of the immunoglobulin superfamily characterized by a single extracellular V-type Ig-like domain. It associates with a homodimer of ITAM motif-containing KARAP / DAP12 adaptor proteins. These adaptor proteins were found to be crucial both for the surface expression of NKp44 and for transducing activating signal. CD336 can mediate triggering of NK cell cytotoxicity as in redirected killing assays. Monoclonal antibody Z231-mediated cross-linking of NKp44 in cloned NK cells resulted in strong lysis of target cells and, Z231-mediated masking of NKp44 partially inhibits cytolytic activity. Such inhibition is greatly increased if an anti-CD335, also known as anti-NKp46 (BAB281), is combined with Z231. The CD336 ligand is still undefined. Since activated NK cells selectively express NKp44, it may play a partial role in the enhanced cytotoxicity of IL2-activated compared to those freshly isolated NK cells from peripheral blood. CD337 ( NKp30 ) CD337, also known as NKp30, is a 30-kDa transmembrane glycoprotein which is a NK-specific triggering receptor involved in non-MHC-restricted natural cytotoxicity. NKp30 is strictly expressed by all resting and activated NK cells including the minor CD3–CD56brightCD16– subset. With NKp46 and NKp44 it is one of the three identified NK natural cytotoxicity receptors (NCR). Its surface expression parallels that of NKp46. NKp46dull NK cells are also characterised by a NKp30dull phenotype while NKp46bright NK cells show a NKp30bright phenotype. The NKp30bright or NKp30dull phenotypes are correlated with high or low cytotoxicity, respectively. CD337 is a member of the immunoglobulin superfamily characterized by a single extracellular V-type Ig-like domain. It is associated with a homodimer of ITAM motif- containing CD3 ζ adaptor proteins. These adaptor proteins are not necessary for surface expression of NKp30, but are necessary for signal transduction. In redirected killing assays, Z25-mediated cross linking of NKp30 induces strong NK cell activation. Conversely, Z25- mediated masking of NKp30 inhibits NK cytotoxicity against target cells. More importantly, CD337 represents the major receptor in induced NK-mediated killing of certain target cells, the lysis of which is largely NKp46 / NKp44 independent. Although the CD337 ligand is unknown, studies report NKp30-mediated lysis of immature dendritic cells (iDC). These findings suggest that DCs express one or more unidentified ligands for CD337. Form Clone: Z231 Isotype: IgG1 Mouse Part# Format Size Status Line Quality Standard PE IOTest IOTest ASR ASR IM3710 A66903 Liquid Liquid 1 mL GMP GMP PC5 0.5 mL

Lysed normal whole blood sample.

NK92 act. IL2 24h Cell line.

Clone: Z25

Isotype: IgG1 Mouse

The Z25 antibody has been used in flow cytometry to analyze the expression of NKp30 on NK cells.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM3709 A66904

1 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

PC5

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 105 US Clinical Diagnostics Catalog