Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CD184 (also known as CXCR4, or Fusin) is a seven-transmembrane domain G protein-coupled surface receptor. Its natural ligand is the stromal cell-derived factor-1 (SDF-1), a C-X-C chemokine which is strongly chemotactic for monocytes and lymphocytes. CD184 has initially been described as the human immunodeficiency virus-1 (HIV-1) entry co-receptor, associated with the surface CD4-gp120 complex before HIV-1 enters target cells. It also acts as an alternative receptor for some isolates of HIV-2. The SDF-1 / CD184 pair may also play a role in the development of the nervous system. Unlike other chemokine receptors, the cellular distribution of CD184 (CXCR4) is nearly ubiquitous; it is expressed on a variety of CD4+ and CD4– cells in the immune system and the central nervous system. In blood, CD184 is highly expressed on neutrophils, monocytes, dendritic cells, NK cells, B cells, and T cells, as well as on platelets. CD184 exhibits a rapid (30 seconds) up-regulation upon activation of lymphocytes, followed by internalization (within 2 minutes). Accordingly, CD184 is detected in large amounts in intracellular stores within leucocytes. Absent from eosinophils and basophils in fresh whole blood, this antigen is inducible ex vivo on eosinophils by glucocorticoids, and on basophils by SDF-1. As a GPCR, CXCR4 binding of SDF-1 triggers certain signaling pathways such as ras, and PI3 kinase. One downstream effect of SDF-1 / CD184 interaction is the activation of transcription factors such as AP-1 and chemokine regulated genes. JAK / STAT pathways also play a role in SDF-1/CXCR4 signaling. CD184 ( CXCR4 ) Isotype: IgG2a Mouse The 12G5 antibody partially inhibits chemotaxis and calcium influx induced by SDF-1. It stains predominantly the naïve, resting CD26 low CD45RA + CD45R0 - T lymphocyte subset of peripheral blood T cells. The 12G5 mAb has been assigned to the CD184 cluster of differentiation during HLDA 7. CD196, also known as CCR6, is G-proteincoupled receptor (GPCR) consisting of an extracellular amino terminal, seven membrane-spanning regions and a cytoplasmic carboxyl terminal. CCR6 is a member of the beta chemokine receptor. CD196 is a 374 amino acids long protein and has a calculated molecular weight of 42 kDa. CD196 is predominantly expressed by B lymphocytes, certain subsets of effector and memory T cells and by immature dendritic cells but not by monocytes, NK cells or granulocytes. CD196 binds CCL20, although members of the β defensin family (family of anti-bacterial peptides) also bind CD196 with a lower affinity. CD196 positive cells, and its ligand CCL20, have been detected in numerous organs, especially the secondary lymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyers patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures. In humans, CCR6 can function to mediate arrest of T cells on dermal endothelial cells and is highly expressed on T cells resident in both healthy and diseased skin. CCR6 and/or CCL20 have been implicated in the pathogenesis of inflammatory joints diseases and inflammatory bowel diseases. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 have a role in inflammatory diseases by recruiting Th17 cells to target tissues. CD196 ( CCR6 ) Clone: 12G5 Part# Format Size Status Line Quality Standard PE IOTest ASR A07409 Liquid 2 mL GMP

Lysed normal whole blood sample. Gated on lymphocytes. Dual staining with CD19-FITC.

Form

Lysed normal whole blood sample.

Clone: B-R35

Isotype: IgG2a Mouse

The B-R35 monoclonal antibody specifically binds to CCR6.

Form

Size

Format

Part#

Status

Line Quality Standard

PC7

B68132

0.5 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 99 Outside US Flow Cytometry Catalog