Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD158b1, CD158b2 and CD158 j molecules are monomeric integral membrane glycoproteins existing in different forms. CD158b1 and CD158b2 (p58.2 and p58.3, respectively) are 58 kDa molecules. CD158j (p50.2) is a 50 kDa molecule. All three have identical extracellular portions, but different transmembrane and cytoplasmic regions. CD158b1 and CD158b2 likely represent allelic forms of the same locus. There is another comprehensive nomenclature which refers to these molecules as KIR2DL2 for CD158b1 (p58.2), KIR2DL3 for CD158b2 (p58.3), and KIR2DS2 for CD158j; these acronyms stand for Killer cell Immunoglobulin (Ig)-like Receptor (KIR), with extracellular part containing 2 Ig Domains (2D), and cytoplasmic tails being either long (L) or short (S). The long intracellular portion of CD158b1/b2 (p58.2/p58.3) makes them inhibitory forms, since they contain two characteristic ITIM motifs (Immunoreceptor Tyrosine-based Inhibition Motif) and are involved in the transduction of an inhibitory signal through the activation of the protein tyrosine phosphatases SHP-1 and/or SHP-2. The stimulatory form CD158h (p50.1) lacks these ITIM motifs. CD158b1/b2 (p58.2/p58.3) function as inhibitory receptors for a group of HLA-C molecules (Cw1, Cw3, Cw7 and Cw8 supertypes). The inhibitory effect is dominant over the activation exerted by the activator form p50.2. The CD158b1/b2 and CD158j molecules are indistinguishable from the extracellular viewpoint. They are expressed by a subset of NK cells, and can be detected on the surface of sub-populations of peripheral T lymphocytes, mostly of CD8+ phenotype. CD158b1/b2,j

Lysed normal whole blood sample.

Clone: GL183 Isotype: IgG1 Mouse The GL183 antibody reacts with both p58.2/p58.3 and p50.2. The antibody restores the ability of p58.2 + NK clones to lyse targets expressing HLA-C molecules.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2278U A66900 A66901 A22333

1 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR RUO

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP GMP

PC5.5

0.5 mL 0.5 mL 0.5 mL 0.2 mg

PC7 APC

UNLB

IM1846

Freeze-dried

-

The CD158e1 and CD158e2 molecules (also known as p70 molecules) are monomeric integral membrane glycoproteins of 70 kDa. CD158e1 and CD158e2 likely represent allelic forms of the same locus of the NKB1 gene. These proteins belong to the family of NK receptors for MHC class I molecules and show a high degree of homology with p58 and p140 receptors. There is another comprehensive nomenclature which refers to these molecules as KIR3DL1 for CD158e1, and KIR3DS1 for CD158e2. These acronyms stand for Killer cell Immunoglobulin (Ig)-like Receptor (KIR), with extracellular part containing 3 Ig Domains (3D), and cytoplasmic tails being either long (L) or short (S). CD158e1 comprises 3 C-type extracellular Ig domains, and two characteristic ITIM motifs (Immunoreceptor Tyrosine-based Inhibition Motif) in the intracellular portion. These motifs are involved in the transduction of an inhibitory signal through the activation of the protein tyrosine phosphatases SHP-1 and/or SHP-2. CD158e1/e2 function as inhibitory receptors for HLA-B molecules of the Bw4 supertype. These molecules are expressed by a subset of NK cells, and can be detected on the surface of sub-populations of peripheral T cells, mostly of CD8+ phenotype. CD158e1/e2

Lysed normal whole blood sample.

Clone: Z27.3.7

Isotype: IgG1 Mouse

The anti-KIR/p70 antibody Z27 restores the ability of p70+ NK clones to lyse HLA-Bw4 targets.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM3292 A60795

1 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

APC

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 94 Outside US Flow Cytometry Catalog