Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD152 antigen, also called CTLA4, is an integral membrane protein belonging to the immunoglobulin superfamily, which is expressed either as a 30 kDa monomer or as a disulfide- linked homodimer. It has homology with the T cell antigen CD28, and like CD28, is a ligand for CD80 and CD86. CTLA-4 is transiently expressed by activated T cells and is not detectable in resting T cells. CD152 and CD28 are involved in the costimulation required for T cell activation. CD152 is an essential checkpoint control for autoimmunity; however, the molecular mechanism (s) by which CTLA-4 achieves its regulatory function are not well understood, especially how it functionally intersects with the CD28 pathway. CD152 ( CTLA-4 )

JURKAT CTLA4 Cell line.

Clone: BNI3 Isotype: IgG2a Mouse The BNI3 antibody can be used to detect intracytoplasmic CD152 (CTLA4) by flow cytometry after permeabilization with IntraPrep Permeabilization Reagent.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2282 IM2070

100 tests

Liquid

RUO RUO

IOTest

GMP GMP

UNLB

0.2 mg

Freeze-dried

-

The CD154 antigen (CD40-ligand), also known as TRAP (Tumor necrosis factor Related Activation Protein), is a member of the Tumor Necrosis Factor (TNF) superfamily. It is a 32 kDa type II transmembrane molecule. CD154 is rapidly induced on T cells (CD4+ and some CD8+ cells) following TCR activation. First identified on activated T cells, CD154 is also expressed on platelets, as well as monocytes and B cells. CD154 can also be shed from activated platelets and lymphocytes in a soluble form (sCD154). This ligand for the CD40 molecule is present on some basophils and mast cells. It has critical regulatory roles in T- and B-lymphocyte function. CD40/CD154 interaction is essential for the development of thymus-dependent humoral immune responses. Ligation of CD40 on B cells by CD154 on T cells promotes B cell proliferation, immunoglobulin (Ig) production, isotype switching and memory B-cell generation. CD154

P3XTBA7 Cell line.

Clone: TRAP-1

Isotype: IgG1 Mouse

The TRAP-1 antibody inhibits activation induced by full-length soluble human CD154.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2216U

2 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 92 Outside US Flow Cytometry Catalog