Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD107a antigen is the heavily glycosylated 110 kDa lysosomal-associated membrane protein, LAMP-1. Together with LAMP-2, they are the mayor glycoproteins on the membrane of lysosome granules. CD107a molecule is ubiquitously found as intracellular antigen. It is also expressed on the surface of activated platelets, PHA activated, a few monocytic cell lines, and fetal thymus stromal cells. It has been described as a marker of cytotoxic CD8+ Tcell degranulation and of NK cell functional activity. As platelet activation marker, it has characteristics of an adhesive molecule. A minor fraction (<2%) of LAMP-1 is associated with the plasma membrane of most nucleated cells, presumably as a result of selective exchange of lysosomal and plasma membranes. Increased surface expression of LAMP-1 has been observed on transformed cells of high metastatic potential, and on embryonic cells. CD107a ( LAMP-1 )

Lysed normal whole blood sample gated on NK cells.

Form Clone: H4A3

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PB

B13978 B12934

0.5 mL 0.5 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

APC

The CD109 antigen is a monomeric glycosyl phosphatidylinositol (GPI)-linked glycoprotein of 170 kDa. It is a novel member of the α 2 macroglobulin ( α 2M) / C3, C4, C5 family of thioester- containing proteins. CD109 is found on vascular endothelial cells, some epithelial cells, activated T-cells, activated platelets, leukemic megakaryoblasts and a subset of bone marrow CD34+ cells. It is not expressed on fresh peripheral blood lymphocytes (PBL). Poorly differentiated (CD34+, TdT+, CD7+) T-acute leukemias and rare cases of chronic myeloid leukemia in megakaryoblast crisis express the CD109 antigen. Furthermore, megakaryoblastoid cell lines (MO7e, MOLM-1) are CD109+. The CD109 antigen may represent a very early marker for hematopoietic cells committed to the megakaryocyte lineage. In contrast, the adult bone marrow CD34+ CD109+ subset identifies the most primitive hematopoietic stem cells capable of long-term culture and lymphoid progenitors of the B-cell lineage. CD109 ( E123 )

KG1A Cell line.

Form Clone: 8A3

Isotype: IgG2a Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

A08933

100 tests

Liquid

RUO

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 84 Outside US Flow Cytometry Catalog