Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CD99 is an heavily O-glycosylated 32-kDa type I transmembrane protein. Its expression has been reported in many cell types, such as hematopoietic cells, endothelial cells, central nervous system ependymal cells, thymocytes, granular cells of the ovary, Sertoli cells, and pancreatic islet cells. It is variably expressed on blood cells: at high density on T cells, NK cells and erythrocytes, at low density on B cells, eosinophils and platelets. Within the functions of CD99 in cells highly expressing this antigen, studies have shown cell death of thymocytes and T lymphocytes, migration through monocyte endothelial junctions by adhesion and diapedesis, cell-cell adhesion in lymphocytes. CD99 has intercellular protein phosphorylation activity, which triggers modulation of T-cell adhesion, induction of homotypic adhesion of corticothymocytes, apoptosis and rosette formation with erythrocytes. CD99

Lysed normal whole blood sample.

Form Clone: HCD99

Isotype: IgG2a Mouse

Size

Format

Part#

Status

Line Quality Standard

ECD APC

B76291 B76270

0.5 mL 0.5 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

The CD103 antigen was originally described as the human mucosal antigen 1 (HML-1) and was subsequently proved to be the integrin α E subunit. CD103 is composed of a 150 kDa chain associated with a 25 kDa chain by a disulfide bond. It is expressed as a heterodimer in association with the integrin β 7 chain. CD103 is expressed on mucosa-associated T lymphocytes and activated cells. This human intra-epithelial lymphocyte marker is expressed in very few resting peripheral blood lymphocytes. α E β 7 binds to E-cadherin on epithelial cells. Activation and TGF β -1 induce CD103 expression. CD103

MOLT16 Cell line.

Clone: 2G5

Isotype: IgG2a Mouse

The 2G5 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC FITC APC

IM1856U B49222 B06204 IM0318

2 mL

Liquid Liquid Liquid

ASR

IOTest IOTest IOTest

GMP

100 tests

CE

98/79/EC

0.5 mL 0.2 mg

ASR RUO

GMP GMP

UNLB

Freeze-dried

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If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 82 Outside US Flow Cytometry Catalog