Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD80 antigen (B7, BB1) is a highly glycosylated single-chain protein. Its extracellular domain consists of two Ig-like domains. This 60 kDa molecule shares with CD86 the capability to be the ligand for two structurally similar molecules expressed on T lymphocytes, CD28 and CD152 (CTLA-4). CD80 antigen is expressed on in vitro activated B lymphocytes after 24h stimulation and at maximal level 48-72h post activation. It is not expressed on the majority of resting B cells from peripheral blood but identifies a subpopulation of B cells that has been previously activated. The antigen is also expressed by HTLV-1 transformed T cells and activated monocytes. CD80 binding provides co-stimulatory signals for T cell activation. CD80

RPMI8866 cell line

Clone: MAB104 Isotype: IgG1 Mouse The MAB104 monoclonal antibody reacts with in vitro activated B lymphocytes, some B cell lines, and weakly with a small proportion of non-activated B cells. This antibody also reacts with activated T cells but not with peripheral monocytes and T cells.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1853U IM1976U B30644 B30642 B30643

2 mL 2 mL

Liquid Liquid Liquid Liquid Liquid

ASR ASR ASR ASR ASR

IOTest IOTest IOTest IOTest IOTest

GMP GMP GMP GMP GMP

PE

PC7 APC

0.5 mL 0.5 mL 0.5 mL

APC-A750

The CD81 molecule is a single chain, 4 transmembrane domain protein (Tetraspan/TM4SF). Both the NH2- and COOH-termini are located inside the cytoplasm, and two loops of the protein sequence are exposed extracellularly. The CD81 antigen is not glycosylated and its molecular weight is 26 kDa. Its tissue distribution is broad. This antigen may be present in some cases as multimolecular complexes, in association with other members of the TM4 superfamily (CD37, CD53) or, on the surface of B cells, in association with CD19 and/or CD21 and/or MHC class II antigens. Most B lymphocytes, at all stages of cellular differentiation, express CD81 at relatively high levels. CD81

Lysed normal whole blood sample.

Clone: JS64 Isotype: IgG2a Mouse The JS64 antibody reacts with the majority of normal lymphocytes, monocytes and eosinophils. Neutrophils and platelets are negative. JS64 has an anti-proliferative effect on some cell lines, but does not cause apoptosis.

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B19717 B25329 IM2579 A87789

0.5 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

FITC

1 mL 2 mL

PE

APC

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 77 Outside US Flow Cytometry Catalog