Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD79a molecule (MB-1, Ig α ) is part of the MB-1/B29 (CD79a/CD79b) disulfide-linked heterodimer which is non-covalently associated with membrane immunoglobulins (Igs) to build the B Cell antigen Receptor (BCR) complex. CD79a is a 40-45 kDa glycoprotein and belongs to the Ig superfamily of surface antigens. CD79a protein is specifically and strictly expressed throughout B lymphocyte differentiation. It appears early in ontogeny, probably before expression of cytoplasmic μ -chain, and is present in the cytoplasm of plasma cells. CD79a

Permeabilized normal whole blood sample.

Clone: HM47 Isotype: IgG1 Mouse The HM47 antibody is recommended to be used in flow cytometry after permeabilization with saponin-based permeabilization reagent IntraPrep. The HM47 antibody reacts with a cytoplasmic epitope of the CD79a molecule.

Form

Size

Format

Part#

Status

Line Quality Standard

PE PE

IM2221

100 tests

Liquid Liquid Liquid Liquid Liquid Liquid

CE

IOTest IOTest IOTest IOTest IOTest IOTest

98/79/EC

IM2221U IM3456U B42018 A60793 B36287

2 mL 1 mL

ASR ASR ASR ASR

GMP GMP GMP GMP

PC5

PC5.5

0.5 mL

APC APC

1 mL

100 tests

CE

98/79/EC

The CD79b molecule (B29, Ig β ) is part of the CD79a / CD79b disulphide-linked heterodimer, non-covalently bound to surface immunoglobulins to form B cell receptors (BCR). The molecular weight of CD79b is about 33 to 40 kDa in reducing conditions. The CD79b molecule expression occurs early in B cells ontogeny. It is restricted to B lymphocytes, first appearing on the surface at the pre-B cell stage and remaining through all stages of B cell differentiation prior to plasma cells. CD79b plays an important role in the regulation of antigen receptor function like the CD3 subunits do with TCR function on T lymphocytes. CD79b

Lysed normal whole blood sample.

Clone: CB3-1 Isotype: IgG1 Mouse The CB3-1 antibody reacts with the external part of the CD79b molecule and is to be used in flow cytometry. This antibody is not suitable for intracytoplasmic staining. For this application the use of the CD79a antibody is recommended.

Form

Size

Format

Part#

Status

Line Quality Standard

PE PE

IM1612

100 tests

Liquid Liquid Liquid Liquid Liquid

CE

IOTest IOTest IOTest IOTest IOTest

98/79/EC

IM1612U B21445 A87784 B96773

2 mL

ASR ASR ASR

GMP GMP GMP

PC5.5

0.5 mL 0.5 mL 50 tests

APC APC

CE

98/79/EC

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 76 Outside US Flow Cytometry Catalog