Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD71 antigen is known as the transferrin receptor. It mediates cellular iron uptake via internalization and recycling. It is a type II membrane glycoprotein, characterized as a disulfide- bound dimer of two identical subunits of 95 kDa each. CD71 is expressed on activated T and B lymphocytes, macrophages and erythroblasts. The N-Terminal, intracytoplasmic domain of CD71 mediates rapid endocytosis and self recycling. CD71 is absent from resting blood leucocytes. Its expression is upregulated on proliferating cells. CD71 is present on reticulocytes and erythroid progenitors in fetal liver, cord blood, and peripheral blood, yet it is lost as these differentiate to mature erythrocytes. CD71 is expressed on marrow stromal cells (MSCs) from bone marrow. CD71

MO7E cell line.

Form Clone: YDJ1.2.2

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

FITC FITC

IM0483 IM0483U IM2001U

100 tests

Liquid Liquid Liquid Liquid Liquid

CE

IOTest IOTest IOTest IOTest IOTest

98/79/EC

2 mL 2 mL

ASR ASR ASR ASR

GMP GMP GMP GMP

PE

APC-A700 APC-A750

A97051 A89313

0.5 mL 0.5 mL

CD73 is a 5’-nucleotidase anchored to the membrane by a glycosylphosphatidylinositol (GPI) structure. It catalyzes the dephosphorylation of purine and pyrimidine ribo- and deoxyribonucleoside mono phosphates to their corresponding nucleosides. CD73 is a dimer of two identical 70 kD subunits. A soluble form of CD73 can be shed from the membrane through proteolytic cleavage or hydrolysis of the GPI anchor by phosphatidylinositol-specific phospholipase. CD73 is expressed on subsets of T and B cells, follicular dendritic cells and on epithelial and endothelial cells. CD73 regulates the availability of adenosine by converting AMP to adenosine. The expression and function of this enzyme are upregulated under hypoxic conditions, as well as by the presence of several pro-inflammatory mediators, such as TGF- β , IFNs, TNF- α , IL-1 β and prostaglandin E2. CD73 can transmit activation signals to T cells and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells. The CD73/adenosine pathway is a potent additional suppressive pathway of Treg cells and also confers a suppressive anti-inflammatory function on the uncommitted primed precursor Thpp cell type. CD73

Lysed normal whole blood sample.

Form Clone: AD-2

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

B68176

1 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 75 Outside US Flow Cytometry Catalog