Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CD66c ( KOR-SA3544 ) The CD66c antigen, also known as non-specific cross-reacting antigen (NCA90), is a granulocyte cell surface GPI-linked protein of 80-100 kDa. It is also called KOR-SA3544. Along with CD66a, CD66b and CD66d, it is a member of the carcinoembryonic (CEA) antigen family which are involved in neutrophil activation and signaling and intercellular adhesion. It is also called CEACAM6. CD66c is a surface (and intracellular) molecule bound to the membrane by a glycosyl- phosphatidylinositol anchor. While its expression on peripheral granulocytes is well recognized, less is known about its distribution in early steps of normal and neoplastic hematopoiesis. It is not expressed by lymphocytes, monocytes, platelets or red blood cells. This antigen is never expressed by normal B cells.

Clone: KOR-SA3544 Isotype: IgG1 Mouse In peripheral blood, the KOR-SA3544 antibody reacts with the majority of granulocytes but not with lymphocytes, monocytes, platelets or red blood cells. In normal bone marrow, only the myeloid cells are positive.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM2039U IM2357U

1 mL 1 mL

Liquid Liquid

ASR ASR

- -

GMP GMP

PE

The CD69 antigen is a 60 kDa disulfide-linked dimeric structure, known as activation inducer molecule (AIM). This structure contains two differentially glycosylated forms (33 and 27 kDa) of a single core protein, that are covalently associated. This activation antigen is one of the earliest appearing cell surface glycoproteins (before other activation antigens such as IL2-R) after T or B-lymphocyte activation but is absent in resting lymphocytes. It is also expressed by activated macrophages, NK cells and other cell types including neutrophils, eosinophils and platelets. Its expression in vitro can be induced by different factors such as PMA, PHA or CD3 monoclonal antibodies. CD69

PHA cell line.

Clone: TP1.55.3 Isotype: IgG2b Mouse The CD69 antibody is useful in combination with CD4, CD8 and CD3 antibodies for T cell activation studies. The TP1.55.3 antibody immunoprecipitates the 60 kDa homodimer and both 27 kDa and 33 kDa subunits from activated peripheral blood lymphocytes under reduced and non-reduced conditions.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM1943U 6607110 IM2656 A80710

2 mL

Liquid Liquid Liquid Liquid Liquid

ASR RUO

IOTest IOTest IOTest IOTest IOTest

GMP GMP

ECD PC5 PC7 APC

100 tests 100 tests

CE

98/79/EC

1 mL 1 mL

ASR ASR

GMP GMP

A80711

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 74 Outside US Flow Cytometry Catalog