Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD65 antigen is analogous to the carbohydrate structure(s) recognized by CD15 antibodies. CD65 is a type II chain fucoganglioside involving the oligosaccharide sequence: Gal β 1-4GlcNac β 1-3Gal β 1-4GlcNac(Fuc α 1-3) β 1-R. CD65 has been suggested as the CD62L ligand, and it has been hypothesized to be involved in the extra vascular infiltration. CD65 is normally expressed on neutrophils, eosinophils, and basophils, on a monocyte subset and on a fraction of CD56bright NK cells. It is not expressed on lymphocytes. CD65 is also found on the membrane of adipocytes and on thymic epithelium but it is not expressed by mastocytes. In myeloid ontogenesis, CD65 seems to be expressed later than myeloperoxidase (MPO). CD65

Lysed normal whole blood sample.

Clone: 88H7

Isotype: IgM Mouse

88H7 antibody was re-assigned from the CD65s to the CD65 cluster during HLDA 6.

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B08164 IM1654U B36299

0.5 mL

Liquid Liquid Liquid

ASR ASR

IOTest IOTest IOTest

GMP GMP

FITC FITC

2 mL

100 tests

CE

98/79/EC

The CD66 antigens were originally described as granulocyte-specific activation antigens, related to the carcino-embryonic antigen (CEA) previously described on colon cancer cells. The CEA/CD66 gene group belongs to the immunoglobulin (Ig) gene superfamily. Among the CD66 cluster, CD66b (also known as CGM6) is a glycosyl-phosphatidylinositol (GPI)-anchored glycoprotein of 95-100 kDa. CD66b is thought to play a role in the regulation of the adhesive activity of CD11/CD18 via signal transduction in neutrophils. It is strongly expressed by myelocytes, metamyelocytes, neutrophils and polymorphonuclear neutrophils. CD66b is weakly expressed by promyelocytes. CD66b

Lysed normal whole blood sample.

Clone: 80H3

Isotype: IgG1 Mouse

The 80H3 antibody does not react with peripheral lymphocytes and monocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC APC

IM0531U B15091 B08756

2 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

GMP GMP GMP

0.5 mL 0.5 mL

APC-A750

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 73 Outside US Flow Cytometry Catalog