Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD62L antigen, also called LECAM-1, L-selectin or leucocyte adhesion molecule 1 [LAM-1], is a 75 kDa molecule, member of the selectin family. As other selectins (CD62E, CD62P), CD62L is a membrane-anchored Ca2+-dependent C-type lectin that binds to cell-surface carbohydrate ligands. CD62L acts as the peripheral lymph node homing receptor. It is also involved in lymphocyte binding to High Endothelial Venules (HEV) and in lymphocyte rolling on activated endothelium. CD62L is expressed by B and T lymphocytes, monocytes, neutrophils, eosinophils, and approximately half of the peripheral NK lymphocytes. It is also expressed by some spleen and bone marrow lymphocytes, as well as by some thymocytes and bone marrow myeloid cells. The expression level of CD62L on lymphocytes may be subject to control mechanisms such as downregulation and/or upregulation. On neutrophils, monocytes and their bone marrow precursors, CD62L is also downregulated by stimulation with granulocyte- macrophage colony stimulating factor (GM-CSF). CD62L Isotype: IgG1 Mouse In contrast to other antibodies recognizing this molecule, DREG56 is particularly effective in blocking CD62L mediated lymphocyte binding to lymph node HEV. DREG56 (ref.33) was used as a CD62L reference monoclonal antibody during the HLDA 6. Clone: DREG56

Lysed normal whole blood sample. Gated on lymphocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B30640 IM1231U IM2214U IM2713U IM2655U B30641 B30639 B26604

0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR ASR ASR ASR ASR ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

GMP GMP GMP GMP GMP GMP GMP GMP

FITC

2 mL 2 mL 1 mL 1 mL

PE

ECD PC5 PC7 APC

0.5 mL 0.5 mL 0.5 mL

APC-A750

The CD62P antigen (P-selectin/GMP-140/PADGEM) is a member of the selectin family. Like other selectins (CD62E and CD62L), CD62P contains an amino terminal lectin-like domain, followed by an EGF domain, nine short consensus repeats (SCR), a transmembrane domain and a short cytoplasmic domain. CD62P (140 kDa) is present in megakaryocytes, in Weibel- Palade bodies of endothelial cells and in α -granules of platelets. CD62P is translocated to the surface membrane upon in vitro and in vivo activation. CD62P is involved in the interaction between activated platelets and with monocytes and neutrophils. CD62P

Platelet rich plasma (PRP) from a normal whole blood sample.

Clone: CLB-Thromb/6 Isotype: IgG1 Mouse The CLB-Thromb/6 antibody recognizes the boundary region between the lectin and EGF-like domains, which is preserved after platelet fixation. It inhibits platelet adhesion to eosinophils in cell adhesion assays.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

A07790 IM1759U

100 tests

Liquid Liquid

CE

IOTest IOTest

98/79/EC

PE

2 mL

ASR

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 71 Outside US Flow Cytometry Catalog